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Literature summary for 2.7.7.50 extracted from

  • Mao, Z.; Joklik, W.K.
    Isolation and enzymatic characterization of protein lambda 2, the reovirus guanylyltransferase (1991), Virology, 185, 377-386.
    View publication on PubMed
Search for more literature for 2.7.7.50

Cloned(Commentary)

Cloned (Comment) Organism
expression in HeLa cells via vaccinia virus strain WR into whose thymidine kinase gene the reovirus lambda2 genome segment has been inserted, possesses neither nucleoside nor RNA 5'-triphosphatase activity nor methyltransferase activity Mammalian orthoreovirus 3

Organism

Organism UniProt Comment Textmining
Mammalian orthoreovirus 3
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Mammalian orthoreovirus 3
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 from infected mouse fibroblasts
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Mammalian orthoreovirus 3 Dearing
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Purification (Commentary)

Purification (Comment) Organism
recombinant from vaccinia virus grown in HeLa cells Mammalian orthoreovirus 3

Storage Stability

Storage Stability Organism
-20°C, 10 mM Tris-HCl buffer, pH 8, 10 mM 2-mercaptoethanol, 50% glycerol, 0.1 mg/ml gelatin, enzyme concentration: 0.1 mg/ml, stable Mammalian orthoreovirus 3

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + pp(5')RNA reversible formation of a covalent intermediate enzyme-GMP Mammalian orthoreovirus 3 G(5')ppp(5')RNA + diphosphate guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue r
GTP + pp(5')RNA reversible formation of a covalent intermediate enzyme-GMP Mammalian orthoreovirus 3 Dearing G(5')ppp(5')RNA + diphosphate guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue r
additional information enzyme also catalyzes GTP-diphosphate exchange Mammalian orthoreovirus 3 ?
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 ?
additional information enzyme also catalyzes GTP-diphosphate exchange Mammalian orthoreovirus 3 Dearing ?
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 ?

Subunits

Subunits Comment Organism
More native enzyme forms a pentamer, recombinant enzyme from vaccinia virus forms a monomer Mammalian orthoreovirus 3