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Literature summary for 2.7.7.50 extracted from

  • Yue, Z.; Maldonado, E.; Pillutla, R.; Cho, H.; Reinberg, D.; Shatkin, A.J.
    Mammalian capping enzyme complements mutant Saccharomyces cerevisiae lacking mRNA guanylyltransferase and selectively binds the elongating form of RNA polymerase II (1997), Proc. Natl. Acad. Sci. USA, 94, 12898-12903.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis Homo sapiens
DNA and amino acid sequence determination and analysis, expression of truncation mutant in Escherichia coli BL21(DE3) and wild-type and mutant K294A enzyme in Saccharomyces haploid deficient mutant strain, functional complementation of the latter by wild-type enzyme Mus musculus
in vitro translation of wild-type and mutant enzyme Mus musculus

Protein Variants

Protein Variants Comment Organism
K294A site-directed mutagenesis, no remaining guanylylation activity, no complementation of a deficient Saccharomyces mutant, RNA 5'-triphosphatase activity is retained Mus musculus
additional information construction of N-terminally truncated mutant consisting of residues 438-597, N-terminal truncation eliminates the RNA 5'-triphosphatase activity Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mus musculus phosphorylated C-terminal domain of RNA polymerase II couples capping to transcription elongation, which results in selective capping of RNA polymerase II transcripts ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens O60942
-
-
Mus musculus O55236
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-

Purification (Commentary)

Purification (Comment) Organism
recombinant truncated mutant, residues 438-597, His-tagged Mus musculus

Reaction

Reaction Comment Organism Reaction ID
GTP + (5')ppPur-mRNA = diphosphate + G(5')pppPur-mRNA conserved KXDG-motif Mus musculus
GTP + (5')ppPur-mRNA = diphosphate + G(5')pppPur-mRNA Lys294 is located in the active site in a KXDG-conserved motif Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + pp(5')RNA
-
Mus musculus G(5')ppp(5')RNA + diphosphate guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue ?
GTP + pp(5')RNA
-
Homo sapiens G(5')ppp(5')RNA + diphosphate guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue ?
additional information enzyme also contains RNA 5'-triphosphatase activity, located at the N-terminus Mus musculus ?
-
?
additional information enzyme also contains RNA 5'-triphosphatase activity, located at the N-terminus Homo sapiens ?
-
?
additional information phosphorylated C-terminal domain of RNA polymerase II couples capping to transcription elongation, which results in selective capping of RNA polymerase II transcripts Mus musculus ?
-
?

Synonyms

Synonyms Comment Organism
capping enzyme
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Mus musculus
capping enzyme
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Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Mus musculus