Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis | Homo sapiens |
DNA and amino acid sequence determination and analysis, expression of truncation mutant in Escherichia coli BL21(DE3) and wild-type and mutant K294A enzyme in Saccharomyces haploid deficient mutant strain, functional complementation of the latter by wild-type enzyme | Mus musculus |
in vitro translation of wild-type and mutant enzyme | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
K294A | site-directed mutagenesis, no remaining guanylylation activity, no complementation of a deficient Saccharomyces mutant, RNA 5'-triphosphatase activity is retained | Mus musculus |
additional information | construction of N-terminally truncated mutant consisting of residues 438-597, N-terminal truncation eliminates the RNA 5'-triphosphatase activity | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mus musculus | phosphorylated C-terminal domain of RNA polymerase II couples capping to transcription elongation, which results in selective capping of RNA polymerase II transcripts | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O60942 | - |
- |
Mus musculus | O55236 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant truncated mutant, residues 438-597, His-tagged | Mus musculus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
GTP + (5')ppPur-mRNA = diphosphate + G(5')pppPur-mRNA | conserved KXDG-motif | Mus musculus | |
GTP + (5')ppPur-mRNA = diphosphate + G(5')pppPur-mRNA | Lys294 is located in the active site in a KXDG-conserved motif | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + pp(5')RNA | - |
Mus musculus | G(5')ppp(5')RNA + diphosphate | guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue | ? | |
GTP + pp(5')RNA | - |
Homo sapiens | G(5')ppp(5')RNA + diphosphate | guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue | ? | |
additional information | enzyme also contains RNA 5'-triphosphatase activity, located at the N-terminus | Mus musculus | ? | - |
? | |
additional information | enzyme also contains RNA 5'-triphosphatase activity, located at the N-terminus | Homo sapiens | ? | - |
? | |
additional information | phosphorylated C-terminal domain of RNA polymerase II couples capping to transcription elongation, which results in selective capping of RNA polymerase II transcripts | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
capping enzyme | - |
Mus musculus |
capping enzyme | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |