Literature summary for 2.7.7.50 extracted from

Hakansson, K.; Wigley, D.B.
Structure of a complex between a cap analog and mRNA guanylyl transferase demonstrates the structural chemistry of RNA capping (1998), Proc. Natl. Acad. Sci. USA, 95, 1505-1510.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
crystals of RNA guanylyltransferase, i.e. capping enzyme, complexed with a mRNA cap analogue G(5')ppp(5')G, enzyme solution: 15 mg/ml, 1.3 mM GpppG, 50 mM Tris, pH 7.5, 0.4 M NaCl, 2 mM EDTA, 4 mM DTT, mixed with equal volume of equilibration solution: potassium phosphate 50 mM, pH 6.5, 5-10% PEG 8000, 2 mM ZnCl2, 24 h, X-ray structure determination and analysis	Paramecium bursaria Chlorella virus 1

Organism

Organism	UniProt	Comment	Textmining
Paramecium bursaria Chlorella virus 1	-	crystal structure: Protein Data Bank 1CKO	-

Purification (Commentary)

Purification (Comment)	Organism
-	Paramecium bursaria Chlorella virus 1

Reaction

Reaction	Comment	Organism	Reaction ID
GTP + (5')ppPur-mRNA = diphosphate + G(5')pppPur-mRNA	mechanism of intermediate formation	Paramecium bursaria Chlorella virus 1	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GTP + pp(5')RNA	-	Paramecium bursaria Chlorella virus 1	G(5')ppp(5')RNA + diphosphate	guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue	?

Synonyms

Synonyms	Comment	Organism
capping enzyme	-	Paramecium bursaria Chlorella virus 1
mRNA guanylyl transferase	-	Paramecium bursaria Chlorella virus 1

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Release 2023.1 (January 2023)