Cloned (Comment) | Organism |
---|---|
gene L2, expression of wild-type and mutant K226A protein lambda2 in Trichoplusia ni or Spodoptera frugiperda SF21 cells via baculovirus infection | Mammalian orthoreovirus 3 |
Protein Variants | Comment | Organism |
---|---|---|
K171A | site-directed mutagenesis, 0.22% of wild-type autoguanylylation activity | Mammalian orthoreovirus 3 |
K190A | site-directed mutagenesis, autoguanylylation inactive | Mammalian orthoreovirus 3 |
K197A | site-directed mutagenesis, 7.5% of wild-type autoguanylylation activity | Mammalian orthoreovirus 3 |
K226A | site-directed mutagenesis, unaltered properties | Mammalian orthoreovirus 3 |
K44A | site-directed mutagenesis, approximately wild-type autoguanylylation activity levels | Mammalian orthoreovirus 3 |
K89A | site-directed mutagenesis, approximately wild-type autoguanylylation activity levels | Mammalian orthoreovirus 3 |
K94A | site-directed mutagenesis, approximately wild-type autoguanylylation activity levels | Mammalian orthoreovirus 3 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
42000 kDa fragment harbors the autoguanylylation activity | Mammalian orthoreovirus 3 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mammalian orthoreovirus 3 | - |
- |
- |
Mammalian orthoreovirus 3 | - |
from infected Trichoplusia ni insect cells via baculovirus | - |
Mammalian orthoreovirus 3 Dearing | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant K226A from Trichoplusia ni or Spodoptera frugiperda SF21 cells via baculovirus infection | Mammalian orthoreovirus 3 |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
GTP + (5')ppPur-mRNA = diphosphate + G(5')pppPur-mRNA | active site | Mammalian orthoreovirus 3 | |
GTP + (5')ppPur-mRNA = diphosphate + G(5')pppPur-mRNA | active site Lys190 | Mammalian orthoreovirus 3 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + pp(5')RNA | GMP is linked to the enzyme via a phosphoamide bond | Mammalian orthoreovirus 3 | G(5')ppp(5')RNA + diphosphate | guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue | r | |
GTP + pp(5')RNA | enzyme can transfer its bound GMP to GDP | Mammalian orthoreovirus 3 | G(5')ppp(5')RNA + diphosphate | guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue | r | |
GTP + pp(5')RNA | formation of a covalent intermediate enzyme-GMP | Mammalian orthoreovirus 3 | G(5')ppp(5')RNA + diphosphate | guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue | r | |
GTP + pp(5')RNA | GMP is linked to the enzyme via a phosphoamide bond | Mammalian orthoreovirus 3 Dearing | G(5')ppp(5')RNA + diphosphate | guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue | r | |
GTP + pp(5')RNA | enzyme can transfer its bound GMP to GDP | Mammalian orthoreovirus 3 Dearing | G(5')ppp(5')RNA + diphosphate | guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue | r | |
GTP + pp(5')RNA | formation of a covalent intermediate enzyme-GMP | Mammalian orthoreovirus 3 Dearing | G(5')ppp(5')RNA + diphosphate | guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue | r | |
additional information | autoguanylylation | Mammalian orthoreovirus 3 | ? | - |
? | |
additional information | autoguanylylation | Mammalian orthoreovirus 3 Dearing | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | 42000 kDa fragment harbors the autoguanylylation activity | Mammalian orthoreovirus 3 |