Cloned (Comment) | Organism |
---|---|
gene A103R, expression of His6-tagged A103R protein in Escherichia coli strain BL21(DE3) | Paramecium bursaria Chlorella virus 1 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
sodium phosphonoformate | foscarnet, inhibition of the formation of the covalent intermediate at 5 mM | Paramecium bursaria Chlorella virus 1 | |
tetrapotassium diphosphate | inhibition of the formation of the covalent intermediate at 5 mM | Paramecium bursaria Chlorella virus 1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic analysis of forward and reverse reactions by real-time fluorescence spectroscopy and radioactive kinetic assays, thermodynamics, overview | Paramecium bursaria Chlorella virus 1 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Paramecium bursaria Chlorella virus 1 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + (5')ppPur-mRNA | Paramecium bursaria Chlorella virus 1 | - |
diphosphate + G(5')pppPur-mRNA | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paramecium bursaria Chlorella virus 1 | - |
gene A103R | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged A103R protein from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Paramecium bursaria Chlorella virus 1 |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
GTP + (5')ppPur-mRNA = diphosphate + G(5')pppPur-mRNA | RNA guanylyltransferase activity is involved in the synthesis of the cap structure found at the 5' end of eukaryotic mRNAs. The RNA guanylyltransferase activity is a two-step ping-pong reaction in which the enzyme first reacts with GTP to produce the enzyme-GMP covalent intermediate with the concomitant release of diphosphate. In the second step of the reaction, the GMP moiety is then transferred to a diphosphorylated RNA. Both reactions are reversible. The second step comprises the limiting steps for both the direct and reverse overall reactions | Paramecium bursaria Chlorella virus 1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + (5')ppPur-mRNA | - |
Paramecium bursaria Chlorella virus 1 | diphosphate + G(5')pppPur-mRNA | - |
r | |
GTP + (5')ppPur-mRNA | two-step reaction, usage of a synthetic RNA substrate of 81 nucleotides from a PCR template encoding the 5' terminal portion of the PBCV-1 DNA polymerase gene, with a T7 RNA polymerase promoter, assay method development and evaluation, overview | Paramecium bursaria Chlorella virus 1 | diphosphate + G(5')pppPur-mRNA | - |
r |
Synonyms | Comment | Organism |
---|---|---|
A103R protein | - |
Paramecium bursaria Chlorella virus 1 |
RNA capping enzyme | - |
Paramecium bursaria Chlorella virus 1 |
RNA guanylyltransferase | - |
Paramecium bursaria Chlorella virus 1 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Paramecium bursaria Chlorella virus 1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Paramecium bursaria Chlorella virus 1 |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetic analysis of both steps of forward and reverse reactions, overview | Paramecium bursaria Chlorella virus 1 |