KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.82 | - |
adenylylsulfate | - |
Auxenochlorella pyrenoidosa | |
10 | - |
ammonia | - |
Auxenochlorella pyrenoidosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
17000 | - |
1 * 26000 + 1 * 21000 + 1 * 17000, SDS-PAGE | Auxenochlorella pyrenoidosa |
21000 | - |
1 * 26000 + 1 * 21000 + 1 * 17000, SDS-PAGE | Auxenochlorella pyrenoidosa |
26000 | - |
1 * 26000 + 1 * 21000 + 1 * 17000, SDS-PAGE | Auxenochlorella pyrenoidosa |
60000 | 65000 | PAGE | Auxenochlorella pyrenoidosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenylylsulfate + NH3 | Escherichia coli | the product adenosine 5'-phosphoramidate replaces AMP as an activator of phosphorylase b, threonine dehydratase and adenylate cyclase | adenosine 5'-phosphoramidate + sulfate | - |
? | |
adenylylsulfate + NH3 | Hordeum vulgare | the product adenosine 5'-phosphoramidate replaces AMP as an activator of phosphorylase b, threonine dehydratase and adenylate cyclase | adenosine 5'-phosphoramidate + sulfate | - |
? | |
adenylylsulfate + NH3 | Spinacia oleracea | the product adenosine 5'-phosphoramidate replaces AMP as an activator of phosphorylase b, threonine dehydratase and adenylate cyclase | adenosine 5'-phosphoramidate + sulfate | - |
? | |
adenylylsulfate + NH3 | Dictyostelium discoideum | the product adenosine 5'-phosphoramidate replaces AMP as an activator of phosphorylase b, threonine dehydratase and adenylate cyclase | adenosine 5'-phosphoramidate + sulfate | - |
? | |
adenylylsulfate + NH3 | Auxenochlorella pyrenoidosa | the product adenosine 5'-phosphoramidate replaces AMP as an activator of phosphorylase b, threonine dehydratase and adenylate cyclase | adenosine 5'-phosphoramidate + sulfate | - |
? | |
adenylylsulfate + NH3 | Euglena sp. | the product adenosine 5'-phosphoramidate replaces AMP as an activator of phosphorylase b, threonine dehydratase and adenylate cyclase | adenosine 5'-phosphoramidate + sulfate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Auxenochlorella pyrenoidosa | - |
- |
- |
Dictyostelium discoideum | - |
- |
- |
Escherichia coli | - |
- |
- |
Euglena sp. | - |
- |
- |
Hordeum vulgare | - |
- |
- |
Spinacia oleracea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Auxenochlorella pyrenoidosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Auxenochlorella pyrenoidosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenylylsulfate + NH3 | very high specificity for the adenylylsulfate and ammonia | Escherichia coli | adenosine 5'-phosphoramidate + sulfate | - |
? | |
adenylylsulfate + NH3 | very high specificity for the adenylylsulfate and ammonia | Hordeum vulgare | adenosine 5'-phosphoramidate + sulfate | - |
? | |
adenylylsulfate + NH3 | very high specificity for the adenylylsulfate and ammonia | Spinacia oleracea | adenosine 5'-phosphoramidate + sulfate | - |
? | |
adenylylsulfate + NH3 | very high specificity for the adenylylsulfate and ammonia | Dictyostelium discoideum | adenosine 5'-phosphoramidate + sulfate | - |
? | |
adenylylsulfate + NH3 | very high specificity for the adenylylsulfate and ammonia | Auxenochlorella pyrenoidosa | adenosine 5'-phosphoramidate + sulfate | - |
? | |
adenylylsulfate + NH3 | very high specificity for the adenylylsulfate and ammonia | Euglena sp. | adenosine 5'-phosphoramidate + sulfate | - |
? | |
adenylylsulfate + NH3 | the product adenosine 5'-phosphoramidate replaces AMP as an activator of phosphorylase b, threonine dehydratase and adenylate cyclase | Escherichia coli | adenosine 5'-phosphoramidate + sulfate | - |
? | |
adenylylsulfate + NH3 | the product adenosine 5'-phosphoramidate replaces AMP as an activator of phosphorylase b, threonine dehydratase and adenylate cyclase | Hordeum vulgare | adenosine 5'-phosphoramidate + sulfate | - |
? | |
adenylylsulfate + NH3 | the product adenosine 5'-phosphoramidate replaces AMP as an activator of phosphorylase b, threonine dehydratase and adenylate cyclase | Spinacia oleracea | adenosine 5'-phosphoramidate + sulfate | - |
? | |
adenylylsulfate + NH3 | the product adenosine 5'-phosphoramidate replaces AMP as an activator of phosphorylase b, threonine dehydratase and adenylate cyclase | Dictyostelium discoideum | adenosine 5'-phosphoramidate + sulfate | - |
? | |
adenylylsulfate + NH3 | the product adenosine 5'-phosphoramidate replaces AMP as an activator of phosphorylase b, threonine dehydratase and adenylate cyclase | Auxenochlorella pyrenoidosa | adenosine 5'-phosphoramidate + sulfate | - |
? | |
adenylylsulfate + NH3 | the product adenosine 5'-phosphoramidate replaces AMP as an activator of phosphorylase b, threonine dehydratase and adenylate cyclase | Euglena sp. | adenosine 5'-phosphoramidate + sulfate | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 1 * 26000 + 1 * 21000 + 1 * 17000, SDS-PAGE | Auxenochlorella pyrenoidosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Auxenochlorella pyrenoidosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
- |
Auxenochlorella pyrenoidosa |