Cloned (Comment) | Organism |
---|---|
phylogenetic analysis, sequence comparison and signature motifs, overview | Trypanosoma brucei |
phylogenetic analysis, sequence comparison and signature motifs, overview | Leishmania tarentolae |
Crystallization (Comment) | Organism |
---|---|
TUT4 in complex with UTP, X-ray diffraction structure analysis | Trypanosoma brucei |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutations of metal coordinating catalytic aspartic residues D66, D68 and D136, render TUT4 inactive but have no effect on UTP binding | Trypanosoma brucei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | TUT3 and TUT4 | Trypanosoma brucei | 5829 | - |
mitochondrion | RET1 | Leishmania tarentolae | 5739 | - |
mitochondrion | TUT1 and TUT2 | Trypanosoma brucei | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
140000 | - |
4 * 140000, TUT1 | Leishmania tarentolae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Trypanosoma brucei | post-transcriptional uridylylation of guide RNAs by RNA editing TUTase 1 or RET1, a multi-functional RNA processing enzyme, and U-insertion mRNA editing by RNA editing TUTase 2 or RET2, biological functions of TUT isozymes, RNA processing in mitochondria of trypanosomes, detailed overview | ? | - |
? | |
additional information | Leishmania tarentolae | post-transcriptional uridylylation of guide RNAs by RNA editing TUTase 1 or RET1, a multi-functional RNA processing enzyme, and U-insertion mRNA editing by RNA editing TUTase 2 or RET2, biological functions of TUT isozymes, RNA processing in mitochondria of trypanosomes, detailed overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania tarentolae | - |
- |
- |
Trypanosoma brucei | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
trypanosomoid form | - |
Trypanosoma brucei | - |
trypanosomoid form | - |
Leishmania tarentolae | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | post-transcriptional uridylylation of guide RNAs by RNA editing TUTase 1 or RET1, a multi-functional RNA processing enzyme, and U-insertion mRNA editing by RNA editing TUTase 2 or RET2, biological functions of TUT isozymes, RNA processing in mitochondria of trypanosomes, detailed overview | Trypanosoma brucei | ? | - |
? | |
additional information | post-transcriptional uridylylation of guide RNAs by RNA editing TUTase 1 or RET1, a multi-functional RNA processing enzyme, and U-insertion mRNA editing by RNA editing TUTase 2 or RET2, biological functions of TUT isozymes, RNA processing in mitochondria of trypanosomes, detailed overview | Leishmania tarentolae | ? | - |
? | |
additional information | UTP recognition mechanism, overview. The NTD-CTD bi-domain catalytic modules shared by TUTases and non-canonical poly(A) polymerases, ncPAPs, are quite promiscuous in NTP binding, NTP selectivity of TUTase-like catalytic modules, RNA specificity, overview | Leishmania tarentolae | ? | - |
? | |
additional information | UTP recognition mechanism, overview. UTP specificity is determined primarily by the two closely positioned carboxylic residues, D297/D421 and E300/E424, which coordinate a crucial water molecule indicated in TUT4/RET2, uracil base interactions with invariant amino acids N147, S148, Y189, D297, E300 of TUT4. The NTD-CTD bi-domain catalytic modules shared by TUTases and non-canonical poly(A) polymerases, ncPAPs, are quite promiscuous in NTP binding, NTP selectivity of TUTase-like catalytic modules, RNA specificity, overview | Trypanosoma brucei | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | in RET1, an extra 150 amino acid insertion constitutes a helix-rich domain essential for activity, the insertion is designated as middle domain, occurs at a conserved site within the catalytic domain and is found in RET1, RET2 and TUT3, but not in TUT4, analysis of Structural diversity of trypanosomal TUTases, overview | Trypanosoma brucei |
tetramer | 4 * 140000, TUT1 | Leishmania tarentolae |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme belongs to a large enzyme superfamily typified by DNA polymerase beta | Trypanosoma brucei |
More | the enzyme belongs to a large enzyme superfamily typified by DNA polymerase beta | Leishmania tarentolae |
RET1 | - |
Trypanosoma brucei |
RET1 | - |
Leishmania tarentolae |
RET2 | - |
Trypanosoma brucei |
terminal RNA uridylyltransferase | - |
Trypanosoma brucei |
TUT1 | - |
Leishmania tarentolae |
TUTase | - |
Trypanosoma brucei |
TUTase | - |
Leishmania tarentolae |
TUTase 1 | - |
Trypanosoma brucei |
TUTase 2 | - |
Trypanosoma brucei |
TUTase 3 | - |
Trypanosoma brucei |
TUTase 4 | - |
Trypanosoma brucei |