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Literature summary for 2.7.7.52 extracted from

  • Ringpis, G.E.; Stagno, J.; Aphasizhev, R.
    Mechanism of U-insertion RNA editing in trypanosome mitochondria: characterization of RET2 functional domains by mutational analysis (2010), J. Mol. Biol., 399, 696-706.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.52

Cloned(Commentary)

Cloned (Comment) Organism
-
 Trypanosoma brucei

Protein Variants

Protein Variants Comment Organism
C83F 230% of wild-type activity for transfer to terminal U residue Trypanosoma brucei
D97A complete inactivation of uridylyl transfer Trypanosoma brucei
K149A mutant retains partial activity Trypanosoma brucei
R141A almost complete inactivation of uridylyl transfer Trypanosoma brucei
R144A almost complete inactivation of uridylyl transfer Trypanosoma brucei
R435A almost complete inactivation of uridylyl transfer Trypanosoma brucei
V271R 10-20% of wild-type activity Trypanosoma brucei
V271R/C83F 240% of wild-type activity for transfer to terminal U residue Trypanosoma brucei
Y319A complete inactivation of uridylyl transfer Trypanosoma brucei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0005
-
 UTP mutant V271R/C83F, cosubstrate RNAn containing a terminal G residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei
0.0006
-
 UTP mutant C83F, cosubstrate RNAn containing a terminal G residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei
0.0025
-
 UTP wild-type, cosubstrate RNAn containing a terminal G residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei
0.0026
-
 UTP mutant C83F, cosubstrate RNAn containing a terminal U residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei
0.0028
-
 UTP mutant V271R, cosubstrate RNAn containing a terminal G residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei
0.003
-
 UTP mutant V271R/C83F, cosubstrate RNAn containing a terminal U residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei
0.0081
-
 UTP mutant K149A, cosubstrate RNAn containing a terminal G residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei
0.0126
-
 UTP wild-type, cosubstrate RNAn containing a terminal U residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei
0.0209
-
 UTP mutant V271R, cosubstrate RNAn containing a terminal U residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UTP + RNAn invariant arginine residues 144 and 435 positioned in the vicinity of the UTP-binding site are critical for isoform RET2 activity on single-stranded and double-stranded RNAs, as well as function in vivo. Recognition of a double-stranded RNA, which resembles a guide RNA/mRNA duplex, is further facilitated by multipoint contacts across the RET2-specific middle domain Trypanosoma brucei diphosphate + RNAn+1
-
 ?
UTP + RNAn containing a terminal G residue substrate dsRNA Trypanosoma brucei diphosphate + RNAn+1
-
 ?
UTP + RNAn containing a terminal U residue substrate dsRNA Trypanosoma brucei diphosphate + RNAn+1
-
 ?

Synonyms

Synonyms Comment Organism
RET2
-
 Trypanosoma brucei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00002
-
 UTP mutant K149A, cosubstrate RNAn containing a terminal G residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei
0.00003
-
 UTP mutant V271R, cosubstrate RNAn containing a terminal G residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei
0.00005
-
 UTP mutant C83F, cosubstrate RNAn containing a terminal G residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei
0.00012
-
 UTP mutant V271R/C83F, cosubstrate RNAn containing a terminal G residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei
0.0003
-
 UTP wild-type, cosubstrate RNAn containing a terminal G residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei
0.0012
-
 UTP mutant V271R, cosubstrate RNAn containing a terminal U residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei
0.0015
-
 UTP mutant C83F, cosubstrate RNAn containing a terminal U residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei
0.0018
-
 UTP mutant V271R/C83F, cosubstrate RNAn containing a terminal U residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei
0.003
-
 UTP wild-type, cosubstrate RNAn containing a terminal U residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0053
-
 UTP mutant K149A, cosubstrate RNAn containing a terminal G residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei
0.0058
-
 UTP mutant V271R, cosubstrate RNAn containing a terminal U residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei
0.0097
-
 UTP mutant V271R, cosubstrate RNAn containing a terminal G residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei
0.0567
-
 UTP mutant C83F, cosubstrate RNAn containing a terminal U residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei
0.0867
-
 UTP mutant C83F, cosubstrate RNAn containing a terminal G residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei
0.117
-
 UTP wild-type, cosubstrate RNAn containing a terminal G residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei
0.21
-
 UTP mutant V271R/C83F, cosubstrate RNAn containing a terminal G residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei
0.25
-
 UTP wild-type, cosubstrate RNAn containing a terminal U residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei
0.6
-
 UTP mutant V271R/C83F, cosubstrate RNAn containing a terminal U residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei