General Stability | Organism |
---|---|
enzyme shows an extreme instability in the presence of salts | Microchaete diplosiphon |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, maximal activity at 10-20 mM | Synechococcus elongatus PCC 7942 = FACHB-805 | |
Mg2+ | required, maximal activity at 10-20 mM | Anabaena sp. | |
Mg2+ | only limited ability to replace Mn2+ | Anabaena cylindrica | |
Mg2+ | 30 mM Mg2+ or 5 mM Mn2+ work equally well for maximal activity | Microchaete diplosiphon | |
Mn2+ | can partially replace Mg2+ in activation | Synechococcus elongatus PCC 7942 = FACHB-805 | |
Mn2+ | can partially replace Mg2+ in activation | Anabaena sp. | |
Mn2+ | 30 mM Mg2+ or 5 mM Mn2+ work equally well for maximal activity | Microchaete diplosiphon | |
Mn2+ | 2-4 mM required for optimal activity | Anabaena cylindrica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
x * 185000 + x * 125000 + x * 47000 + x * 30000 + x * 93000, SDS-PAGE | Anabaena cylindrica |
38000 | - |
x * 190000 + x * 145000 + x * 72000 + x * 38000, SDS-PAGE | Synechococcus elongatus PCC 7942 = FACHB-805 |
41000 | - |
x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE | Synechococcus elongatus PCC 7942 = FACHB-805 |
41000 | - |
x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE | Anabaena sp. |
41000 | - |
x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE | Anabaena cylindrica |
41000 | - |
x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE | Microchaete diplosiphon |
41000 | - |
x * 161000 + x * 134000 + x * 72000 + x * 41000 + x * 91000, SDS-PAGE | Microchaete diplosiphon |
47000 | - |
x * 185000 + x * 125000 + x * 47000 + x * 30000 + x * 93000, SDS-PAGE | Anabaena cylindrica |
52000 | - |
x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE | Synechococcus elongatus PCC 7942 = FACHB-805 |
52000 | - |
x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE | Anabaena sp. |
52000 | - |
x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE | Anabaena cylindrica |
52000 | - |
x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE | Microchaete diplosiphon |
66000 | - |
x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE | Synechococcus elongatus PCC 7942 = FACHB-805 |
66000 | - |
x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE | Anabaena sp. |
66000 | - |
x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE | Anabaena cylindrica |
66000 | - |
x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE | Microchaete diplosiphon |
72000 | - |
x * 161000 + x * 134000 + x * 72000 + x * 41000 + x * 91000, SDS-PAGE | Microchaete diplosiphon |
72000 | - |
x * 190000 + x * 145000 + x * 72000 + x * 38000, SDS-PAGE | Synechococcus elongatus PCC 7942 = FACHB-805 |
91000 | - |
x * 161000 + x * 134000 + x * 72000 + x * 41000 + x * 91000, SDS-PAGE | Microchaete diplosiphon |
93000 | - |
x * 185000 + x * 125000 + x * 47000 + x * 30000 + x * 93000, SDS-PAGE | Anabaena cylindrica |
124000 | - |
x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE | Synechococcus elongatus PCC 7942 = FACHB-805 |
124000 | - |
x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE | Anabaena sp. |
124000 | - |
x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE | Anabaena cylindrica |
124000 | - |
x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE | Microchaete diplosiphon |
125000 | - |
x * 185000 + x * 125000 + x * 47000 + x * 30000 + x * 93000, SDS-PAGE | Anabaena cylindrica |
134000 | - |
x * 161000 + x * 134000 + x * 72000 + x * 41000 + x * 91000, SDS-PAGE | Microchaete diplosiphon |
145000 | - |
x * 190000 + x * 145000 + x * 72000 + x * 38000, SDS-PAGE | Synechococcus elongatus PCC 7942 = FACHB-805 |
161000 | - |
x * 161000 + x * 134000 + x * 72000 + x * 41000 + x * 91000, SDS-PAGE | Microchaete diplosiphon |
171000 | - |
x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE | Synechococcus elongatus PCC 7942 = FACHB-805 |
171000 | - |
x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE | Anabaena sp. |
171000 | - |
x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE | Anabaena cylindrica |
171000 | - |
x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE | Microchaete diplosiphon |
185000 | - |
x * 185000 + x * 125000 + x * 47000 + x * 30000 + x * 93000, SDS-PAGE | Anabaena cylindrica |
190000 | - |
x * 190000 + x * 145000 + x * 72000 + x * 38000, SDS-PAGE | Synechococcus elongatus PCC 7942 = FACHB-805 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Anabaena cylindrica | - |
- |
- |
Anabaena sp. | - |
PCC 7120 | - |
Microchaete diplosiphon | - |
- |
- |
Synechococcus elongatus PCC 7942 = FACHB-805 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
PCC 7120 | Anabaena sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0156 | - |
- |
Anabaena sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nucleoside triphosphate + RNAn | the enzyme requires DNA as template | Synechococcus elongatus PCC 7942 = FACHB-805 | diphosphate + RNAn+1 | - |
? | |
nucleoside triphosphate + RNAn | the enzyme requires DNA as template | Anabaena sp. | diphosphate + RNAn+1 | - |
? | |
nucleoside triphosphate + RNAn | the enzyme requires DNA as template | Anabaena cylindrica | diphosphate + RNAn+1 | - |
? | |
nucleoside triphosphate + RNAn | the enzyme requires DNA as template | Microchaete diplosiphon | diphosphate + RNAn+1 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE | Synechococcus elongatus PCC 7942 = FACHB-805 |
? | x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE | Anabaena sp. |
? | x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE | Anabaena cylindrica |
? | x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE | Microchaete diplosiphon |
? | x * 161000 + x * 134000 + x * 72000 + x * 41000 + x * 91000, SDS-PAGE | Microchaete diplosiphon |
? | x * 190000 + x * 145000 + x * 72000 + x * 38000, SDS-PAGE | Synechococcus elongatus PCC 7942 = FACHB-805 |
? | x * 185000 + x * 125000 + x * 47000 + x * 30000 + x * 93000, SDS-PAGE | Anabaena cylindrica |