Inhibitors | Comment | Organism | Structure |
---|---|---|---|
rifampicin | 0.00006 mg/ml, 50% inactivation | Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | maximal activity in presence of 5-10 mM MgCl2 | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
betax,betax,alphax,sigmax, x * 156000 + x * 151000 + x * 87000 + x * 42000, SDS-PAGE | Pseudomonas putida |
87000 | - |
betax,betax,alphax,sigmax, x * 156000 + x * 151000 + x * 87000 + x * 42000, SDS-PAGE | Pseudomonas putida |
151000 | - |
betax,betax,alphax,sigmax, x * 156000 + x * 151000 + x * 87000 + x * 42000, SDS-PAGE | Pseudomonas putida |
156000 | - |
betax,betax,alphax,sigmax, x * 156000 + x * 151000 + x * 87000 + x * 42000, SDS-PAGE | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
PpY101 | - |
Pseudomonas putida PpY101 | - |
PpY101 | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nucleoside triphosphate + RNAn | the enzyme requires DNA as template | Pseudomonas putida | diphosphate + RNAn+1 | - |
? | |
nucleoside triphosphate + RNAn | the enzyme requires DNA as template | Pseudomonas putida PpY101 | diphosphate + RNAn+1 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | betax,betax,alphax,sigmax, x * 156000 + x * 151000 + x * 87000 + x * 42000, SDS-PAGE | Pseudomonas putida |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 50 | 37°C: about 50% of maximal activity, 50°C: about 80% of maximal activity | Pseudomonas putida |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
10 min, stable | Pseudomonas putida |
40 | - |
10 min, slight inactivation | Pseudomonas putida |
50 | - |
10 min, slight inactivation | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pseudomonas putida |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | pH 7.0: about 30% of maximal activity, pH 9.0: about 60% of maximal activity | Pseudomonas putida |