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Literature summary for 2.7.7.6 extracted from

  • Azuma, Y.; Yamagishi, M.; Ishihama, A.
    Subunits of the Schizosaccharomyces pombe RNA polymerase II: enzyme purification and structure of the subunit 3 gene (1993), Nucleic Acids Res., 21, 3749-3754.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
subunit 3 gene, rpb3 Schizosaccharomyces pombe

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
13000
-
x * 210000 + x * 150000 + x * 40000 + x * 34000 + x * 33000 + x * 25000 + x * 20000 + x * 16000 + x * 15000 + x * 13500 + x * 13000, the enzyme contains more than eleven polypeptides, SDS-PAGE Schizosaccharomyces pombe
13500
-
x * 210000 + x * 150000 + x * 40000 + x * 34000 + x * 33000 + x * 25000 + x * 20000 + x * 16000 + x * 15000 + x * 13500 + x * 13000, the enzyme contains more than eleven polypeptides, SDS-PAGE Schizosaccharomyces pombe
15000
-
x * 210000 + x * 150000 + x * 40000 + x * 34000 + x * 33000 + x * 25000 + x * 20000 + x * 16000 + x * 15000 + x * 13500 + x * 13000, the enzyme contains more than eleven polypeptides, SDS-PAGE Schizosaccharomyces pombe
16000
-
x * 210000 + x * 150000 + x * 40000 + x * 34000 + x * 33000 + x * 25000 + x * 20000 + x * 16000 + x * 15000 + x * 13500 + x * 13000, the enzyme contains more than eleven polypeptides, SDS-PAGE Schizosaccharomyces pombe
20000
-
x * 210000 + x * 150000 + x * 40000 + x * 34000 + x * 33000 + x * 25000 + x * 20000 + x * 16000 + x * 15000 + x * 13500 + x * 13000, the enzyme contains more than eleven polypeptides, SDS-PAGE Schizosaccharomyces pombe
25000
-
x * 210000 + x * 150000 + x * 40000 + x * 34000 + x * 33000 + x * 25000 + x * 20000 + x * 16000 + x * 15000 + x * 13500 + x * 13000, the enzyme contains more than eleven polypeptides, SDS-PAGE Schizosaccharomyces pombe
33000
-
x * 210000 + x * 150000 + x * 40000 + x * 34000 + x * 33000 + x * 25000 + x * 20000 + x * 16000 + x * 15000 + x * 13500 + x * 13000, the enzyme contains more than eleven polypeptides, SDS-PAGE Schizosaccharomyces pombe
34000
-
x * 210000 + x * 150000 + x * 40000 + x * 34000 + x * 33000 + x * 25000 + x * 20000 + x * 16000 + x * 15000 + x * 13500 + x * 13000, the enzyme contains more than eleven polypeptides, SDS-PAGE Schizosaccharomyces pombe
40000
-
x * 210000 + x * 150000 + x * 40000 + x * 34000 + x * 33000 + x * 25000 + x * 20000 + x * 16000 + x * 15000 + x * 13500 + x * 13000, the enzyme contains more than eleven polypeptides, SDS-PAGE Schizosaccharomyces pombe
150000
-
x * 210000 + x * 150000 + x * 40000 + x * 34000 + x * 33000 + x * 25000 + x * 20000 + x * 16000 + x * 15000 + x * 13500 + x * 13000, the enzyme contains more than eleven polypeptides, SDS-PAGE Schizosaccharomyces pombe
210000
-
x * 210000 + x * 150000 + x * 40000 + x * 34000 + x * 33000 + x * 25000 + x * 20000 + x * 16000 + x * 15000 + x * 13500 + x * 13000, the enzyme contains more than eleven polypeptides, SDS-PAGE Schizosaccharomyces pombe

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe
-
-
-

Purification (Commentary)

Purification (Comment) Organism
RNA polymerase II Schizosaccharomyces pombe

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.6
-
-
Schizosaccharomyces pombe

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nucleoside triphosphate + RNAn the enzyme requires DNA as template Schizosaccharomyces pombe diphosphate + RNAn+1
-
?

Subunits

Subunits Comment Organism
? x * 210000 + x * 150000 + x * 40000 + x * 34000 + x * 33000 + x * 25000 + x * 20000 + x * 16000 + x * 15000 + x * 13500 + x * 13000, the enzyme contains more than eleven polypeptides, SDS-PAGE Schizosaccharomyces pombe