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Literature summary for 2.7.7.6 extracted from

  • Paschal, B.M.; McReynolds, L.A.; Noren, C.J.; Nichols, N.M.
    RNA polymerases (2008), Curr. Protoc. Mol. Biol., Chapter 3, Unit3.8.
    View publication on PubMed

Application

Application Comment Organism
synthesis the enzyme is useful for in vitro transcription reactions to produce preparative quantities of transcribed RNA and labeled RNA probes, method evaluation, overview Escherichia coli
synthesis the enzyme is useful for in vitro transcription reactions to produce preparative quantities of transcribed RNA and labeled RNA probes, transcripts thousands of nucleotides in length, as are the major applications of these reactions, method evaluation, overview. Phage T7 RNA polymerase is an extremely processive enzyme in high-yield transcription of DNA sequences inserted downstream from the corresponding T7 promoter, applications overview Escherichia phage T7
synthesis the enzyme is useful for in vitro transcription reactions to produce preparative quantities of transcribed RNAand labeled RNA probes, transcripts thousands of nucleotides in length, as are the major applications of these reactions, method evaluation, overview. Phage SP6 RNA polymerase is an extremely processive enzyme in high-yield transcription of DNA sequences inserted downstream from the corresponding SP6 promoter, applications overview Zindervirus SP6
synthesis the enzyme is useful for in vitro transcription reactions to produce preparative quantities of transcribed RNAand labeled RNA probes, transcripts thousands of nucleotides in length, as are the major applications of these reactions, method evaluation, overview. Phage T3 RNA polymerase is an extremely processive enzyme in high-yield transcription of DNA sequences inserted downstream from the corresponding T3 promoter, applications overview Enterobacteria phage T3

Cloned(Commentary)

Cloned (Comment) Organism
-
Escherichia coli
construction of plasmids with polylinker cloning sites adjacent to the RNA polymerase promoter Escherichia phage T7
construction of plasmids with polylinker cloning sites adjacent to the RNA polymerase promoter Enterobacteria phage T3
construction of plasmids with polylinker cloning sites adjacent to the RNA polymerase promoter Zindervirus SP6

Metals/Ions

Metals/Ions Comment Organism Structure
K+ activates Escherichia coli
Mg2+ required Escherichia coli
Mg2+ required Escherichia phage T7
Mg2+ required Enterobacteria phage T3
Mg2+ required Zindervirus SP6

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
480000
-
about Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nucleoside triphosphate + RNAn Escherichia coli template is DNA diphosphate + RNAn+1
-
?
nucleoside triphosphate + RNAn Escherichia phage T7 template is DNA diphosphate + RNAn+1
-
?
nucleoside triphosphate + RNAn Enterobacteria phage T3 template is DNA diphosphate + RNAn+1
-
?
nucleoside triphosphate + RNAn Zindervirus SP6 template is DNA diphosphate + RNAn+1
-
?

Organism

Organism UniProt Comment Textmining
Enterobacteria phage T3
-
-
-
Escherichia coli
-
-
-
Escherichia phage T7
-
-
-
Zindervirus SP6
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the core enzyme, which lacks the sigma subunit, synthesizes short transcripts relatively uniformly on the DNA template in the presence of high concentrations of random primers and low NTP concentrations Escherichia coli ?
-
?
nucleoside triphosphate + RNAn template is DNA Escherichia coli diphosphate + RNAn+1
-
?
nucleoside triphosphate + RNAn template is DNA Escherichia phage T7 diphosphate + RNAn+1
-
?
nucleoside triphosphate + RNAn template is DNA Enterobacteria phage T3 diphosphate + RNAn+1
-
?
nucleoside triphosphate + RNAn template is DNA Zindervirus SP6 diphosphate + RNAn+1
-
?
nucleoside triphosphate + RNAn template is DNA, native or denatured, method evaluation: specificity and extent of transcription depends strongly on the quality of the DNA preparation, the strength of the promoter and terminator sequences, and the kind and concentration of mono- and divalent cations in the reaction mixture Escherichia coli diphosphate + RNAn+1
-
?

Subunits

Subunits Comment Organism
pentamer subunits structure alpha2betabeta'sigmaomega Escherichia coli

Synonyms

Synonyms Comment Organism
DNA-dependent RNA polymerase
-
Escherichia coli
DNA-dependent RNA polymerase
-
Escherichia phage T7
DNA-dependent RNA polymerase
-
Enterobacteria phage T3
DNA-dependent RNA polymerase
-
Zindervirus SP6

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli
37
-
assay at Escherichia phage T7
37
-
assay at Enterobacteria phage T3
37
-
assay at Zindervirus SP6

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Escherichia phage T7
7.5
-
assay at Enterobacteria phage T3
7.5
-
assay at Zindervirus SP6
8
-
assay at Escherichia coli