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Literature summary for 2.7.7.7 extracted from
- The isolation and properties of DNA polymerase II from Escherichia coli (1974), Methods Enzymol., 29, 13-22.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Escherichia coli |  |
| DTT | - |
Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 130000 | - |
gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxynucleoside triphosphate + DNAn | Escherichia coli | polymerase II: role in DNA repair | diphosphate + DNAn+1 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
pol II | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| polymerase II | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.066 | - |
polymerase II | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxynucleoside triphosphate + DNAn | template specificity of polymerase II | Escherichia coli | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | polymerase II: role in DNA repair | Escherichia coli | diphosphate + DNAn+1 | - |
? | |
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