Cloned (Comment) | Organism |
---|---|
mutant proteins expressed from baculovirus constructs in insect cells | Drosophila melanogaster |
Protein Variants | Comment | Organism |
---|---|---|
DELTA413470 | mutation in the spacer region of the alpha-subunit. Obtained in small amounts due to low solubility, mutant has barely detectable DNA polymerase activity | Drosophila melanogaster |
DELTA483533 | expressed efficiently and purified as soluble holoenzyme complex associated with wild-type beta-subunit. The DNA polymerase activity of the mutant holoenzyme is reduced greatly as compared to wild type | Drosophila melanogaster |
DELTA536581 | expressed efficiently and purified as soluble holoenzyme complex associated with wild-type beta-subunit. The DNA polymerase activity of the mutant enzyme is 80% of wild-type holoenzyme | Drosophila melanogaster |
DELTA666742 | mutation in the spacer region of the alpha-subunit. Obtained in small amounts due to low solubility, mutant has barely detectable DNA polymerase activity | Drosophila melanogaster |
F576A | mutation in alpha-subunit, mutant enzyme retains about 50% of wild-type activity | Drosophila melanogaster |
G575A | mutation in alpha-subunit, mutant enzyme has nearly normal activity | Drosophila melanogaster |
G575A/W576A/F578A | mutation in alpha-subunit completely reduces DNA polymerase activity | Drosophila melanogaster |
K557A | mutation in alpha-subunit, mutant enzyme has nearly normal activity | Drosophila melanogaster |
K687A/D688A/F689A | mutation in alpha-subunit resulted in DNA polymerase activities that is 6080% of wild-type enzyme | Drosophila melanogaster |
L558A | mutation in alpha-subunit, mutant enzyme retains about 50% of wild-type activity | Drosophila melanogaster |
P556A | mutation in alpha-subunit, mutant enzyme has nearly normal activity | Drosophila melanogaster |
P556A/K557A/L558A | mutation in alpha-subunit completely reduces DNA polymerase activity | Drosophila melanogaster |
S719A/Y720A/W721 | mutation in alpha-subunit completely reduces DNA polymerase activity | Drosophila melanogaster |
W576A | mutation in alpha-subunit, mutant enzyme is nearly inactive | Drosophila melanogaster |
Y419A/E420A/D421A | mutation in alpha-subunit resulted in DNA polymerase activities that is 6080% of wild-type enzyme | Drosophila melanogaster |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Drosophila melanogaster | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant mutant proteins | Drosophila melanogaster |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | - |
Drosophila melanogaster | diphosphate + DNAn+1 | - |
? |