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Literature summary for 2.7.7.7 extracted from
- Analysis of processivity of mungbean dideoxynucleotide-sensitive DNA polymerase and detection of the activity and expression of the enzyme in the meristematic and meiotic tissues and following DNA damaging agent (2008), Arch. Biochem. Biophys., 475, 55-65.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ATP | considerable enhancement in processivity at 1 mM ATP | Vigna radiata |  |
| spermidine | considerable enhancement in processivity at 2-4 mM spermidine | Vigna radiata | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ddTTP | processivity is strongly inhibited in the presence of 0.01 and 0.02 mM ddTTP | Vigna radiata | |
| Mg2+ | Mg2+ concentrations beyond 6 mM are inhibitory in activity assay | Vigna radiata | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | optimal processivity at 100-150 mM | Vigna radiata | |
| Mg2+ | optimal processivity at 6 mM, no synthesis is observed in the absence of Mg2+ | Vigna radiata | |
| Mn2+ | - |
Vigna radiata | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62000 | - |
SDS-PAGE | Vigna radiata |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vigna radiata | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Vigna radiata | - |
| seedling | active in both meristematic and meiotic tissues | Vigna radiata | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxynucleoside triphosphate + DNAn | - |
Vigna radiata | diphosphate + DNAn+1 | - |
? | |
| additional information | the enzyme is unable to use single stranded DNA or double stranded blunt end DNA | Vigna radiata | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ddNTP-sensitive DNA polymerase | - |
Vigna radiata |
| DNA pol | - |
Vigna radiata |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Vigna radiata |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 48 | - |
activity is almost completely lost above 48°C | Vigna radiata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Vigna radiata |
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