Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta(DE3) cells | Thermococcus marinus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | highly dependent on MgCl2 in the range of 0-20 mM with maximal activity at 14 mM MgCl2 and no detectable activity in the absence of MgCl2 | Thermococcus marinus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
about 90000 Da, mature DNA polymerase, SDS-PAGE | Thermococcus marinus |
152400 | - |
about 90000 Da, DNA polymerase precursor form, estimated from amino acid sequence | Thermococcus marinus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus marinus | C7AIP4 | strain DSM 15227 | - |
Purification (Comment) | Organism |
---|---|
HiTrap heparin column chromatography, gel filtration | Thermococcus marinus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | - |
Thermococcus marinus | diphosphate + DNAn+1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DNA polymerase | - |
Thermococcus marinus |
Pol I | - |
Thermococcus marinus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
- |
Thermococcus marinus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
94 | 99 | the half life of the enzyme is about 2 h at 94°C and 45 min at 99°C | Thermococcus marinus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
in Tris-HCl buffer | Thermococcus marinus |
General Information | Comment | Organism |
---|---|---|
physiological function | the presence of 5'->3' exonuclease activity in DNA polymerase can replace mismatched base pairs with the correct nucleotide, DNA polymerase possesses 3'->5' exonuclease activity | Thermococcus marinus |