Cloned (Comment) | Organism |
---|---|
gene polA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli strain JM109(DE3) | Geobacillus kaue |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | polI activity requires the presence of monovalent ions, above 100 mM, monovalent salts become inhibitory for the activity | Geobacillus kaue |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | required, optimal at 85 mM | Geobacillus kaue | |
Mg2+ | polI activity is absolutely dependent on the presence of divalent cations Mg2+ and Mn2+, Mn2+ cannot be replaced with Mg2+ completely, optimal at 8 mM | Geobacillus kaue | |
Mn2+ | polI activity is absolutely dependent on the presence of divalent cations Mg2+ and Mn2+, Mn2+ cannot be replaced with Mg2+ completely | Geobacillus kaue | |
additional information | polI activity requires the presence of monovalent ions, above 100 mM, monovalent salts become inhibitory for the activity | Geobacillus kaue | |
NaCl | required, optimal at 100 mM | Geobacillus kaue | |
NH4Cl | required, optimal at 80 mM | Geobacillus kaue |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
99000 | - |
x * 99000, about, sequence calcualtion | Geobacillus kaue |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus kaue | B6E9X1 | isolated from hot springs in Gönen and Hisaralan, Turkey, gene polA | - |
Geobacillus kaue NB | B6E9X1 | isolated from hot springs in Gönen and Hisaralan, Turkey, gene polA | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged polI from Escherichia coli strain JM109(DE3) by nickel affinity chromatography | Geobacillus kaue |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
37 | - |
recombinant purified detagged enzyme, pH 7.5, 37°C | Geobacillus kaue |
Subunits | Comment | Organism |
---|---|---|
? | x * 99000, about, sequence calcualtion | Geobacillus kaue |
More | three-dimensional structure prediction using homology modeling. The structure for DNA polymerase domain (residues 471-878) of Gkaue polI is depicted resembling a right hand with fingers, palm, and thumb subdomains | Geobacillus kaue |
Synonyms | Comment | Organism |
---|---|---|
DNA deoxynucleotidyltransferase | - |
Geobacillus kaue |
DNA polymerase I | - |
Geobacillus kaue |
polI | - |
Geobacillus kaue |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Geobacillus kaue |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
purified recombinant enzyme, 15 min, loss of 50% activity | Geobacillus kaue |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | - |
Geobacillus kaue |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | activity range, activity declines sharply above pH 9.0 | Geobacillus kaue |
General Information | Comment | Organism |
---|---|---|
evolution | DNA polymerase I belongs to the DNA polymerase family A, all the functionally important regions in the polymerase active site of Geobacillus kaue polI are conserved, phylogenetic analysis, evolutionary relationship of diverse Geobacillus species, overview | Geobacillus kaue |