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Literature summary for 2.7.7.7 extracted from
- Biochemical, cellular and molecular identification of DNA polymerase alpha in yeast mitochondria (2013), Biochimie, 95, 759-771.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene POL1 encoding mitochondrial DNA pol NI or DNA polymerase alpha catalytic subunit A/DNA polymerase subunit alphabeta, POL12, DNA and amino acid sequence determination and analysis, expression as GFP-tagged enzyme in yeast strain BY4741 | Saccharomyces cerevisiae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | in yeast mitochondria two different DNA polymerases exist, pol gamma and pol NI, i.e. DNA polymerase alpha catalytic subunit A and its associated DNA polymerase subunit alphabeta, POL12 | Saccharomyces cerevisiae | 5739 | - |
| mitoplast | DNA pol NI | Saccharomyces cerevisiae | - |
- |
| soluble | DNA pol NI | Saccharomyces cerevisiae | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Saccharomyces cerevisiae |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxynucleoside triphosphate + DNAn | Saccharomyces cerevisiae | - |
diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | Saccharomyces cerevisiae BY4742 | - |
diphosphate + DNAn+1 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | P13382 | gene MIP1 encoding DNA pol gamma, and gene POL1 encoding DNA polymerase alpha catalytic subunit A | - |
| Saccharomyces cerevisiae BY4742 | P13382 | gene MIP1 encoding DNA pol gamma, and gene POL1 encoding DNA polymerase alpha catalytic subunit A | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native DNA pol NI or DNA polymerase alpha catalytic subunit A/DNA polymerase subunit alphabeta, POL12, 1700fold from mitochondria by cation and anion exchange chromatography, heparin affinity chromatography, and gel filtration | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.22 | - |
purified DNA pol NI, pH 7.5, 37°C | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxynucleoside triphosphate + DNAn | - |
Saccharomyces cerevisiae | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | - |
Saccharomyces cerevisiae BY4742 | diphosphate + DNAn+1 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DNA pol NI | - |
Saccharomyces cerevisiae |
| mtDNA polymerase NI | - |
Saccharomyces cerevisiae |
| pol NI | - |
Saccharomyces cerevisiae |
| POL1 | - |
Saccharomyces cerevisiae |
| polymerase alpha catalytic subunit A | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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