Cloned (Comment) | Organism |
---|---|
gene POL1 encoding mitochondrial DNA pol NI or DNA polymerase alpha catalytic subunit A/DNA polymerase subunit alphabeta, POL12, DNA and amino acid sequence determination and analysis, expression as GFP-tagged enzyme in yeast strain BY4741 | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | in yeast mitochondria two different DNA polymerases exist, pol gamma and pol NI, i.e. DNA polymerase alpha catalytic subunit A and its associated DNA polymerase subunit alphabeta, POL12 | Saccharomyces cerevisiae | 5739 | - |
mitoplast | DNA pol NI | Saccharomyces cerevisiae | - |
- |
soluble | DNA pol NI | Saccharomyces cerevisiae | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | Saccharomyces cerevisiae | - |
diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | Saccharomyces cerevisiae BY4742 | - |
diphosphate + DNAn+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P13382 | gene MIP1 encoding DNA pol gamma, and gene POL1 encoding DNA polymerase alpha catalytic subunit A | - |
Saccharomyces cerevisiae BY4742 | P13382 | gene MIP1 encoding DNA pol gamma, and gene POL1 encoding DNA polymerase alpha catalytic subunit A | - |
Purification (Comment) | Organism |
---|---|
native DNA pol NI or DNA polymerase alpha catalytic subunit A/DNA polymerase subunit alphabeta, POL12, 1700fold from mitochondria by cation and anion exchange chromatography, heparin affinity chromatography, and gel filtration | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.22 | - |
purified DNA pol NI, pH 7.5, 37°C | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | - |
Saccharomyces cerevisiae | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | - |
Saccharomyces cerevisiae BY4742 | diphosphate + DNAn+1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DNA pol NI | - |
Saccharomyces cerevisiae |
mtDNA polymerase NI | - |
Saccharomyces cerevisiae |
pol NI | - |
Saccharomyces cerevisiae |
POL1 | - |
Saccharomyces cerevisiae |
polymerase alpha catalytic subunit A | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Saccharomyces cerevisiae |