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Literature summary for 2.7.7.7 extracted from

  • Dietrich, J.; Schmitt, P.; Zieger, M.; Preve, B.; Rolland, J.L.; Chaabihi, H.; Gueguen, Y.
    PCR performance of the highly thermostable proof-reading B-type DNA polymerase from Pyrococcus abyssi (2002), FEMS Microbiol. Lett., 217, 89-94.
    View publication on PubMed
Search for more literature for 2.7.7.7

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Pyrococcus abyssi

Organism

Organism UniProt Comment Textmining
Pyrococcus abyssi
-
-
-
Pyrococcus abyssi Orsay
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrococcus abyssi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn PCR performance and fidelity parameters are highest in the presence of 20 mM Tris-HCl, pH 9.0, 1.5 mM MgSO4, 25 mM KCl, 10 mM (NH4)2SO4 and 40 microM of each dNTP. Under these conditions, the error rate is 0.66.10(-6) mutations/nucleotide/duplication Pyrococcus abyssi diphosphate + DNAn+1
-
 ?
deoxynucleoside triphosphate + DNAn PCR performance and fidelity parameters are highest in the presence of 20 mM Tris-HCl, pH 9.0, 1.5 mM MgSO4, 25 mM KCl, 10 mM (NH4)2SO4 and 40 microM of each dNTP. Under these conditions, the error rate is 0.66.10(-6) mutations/nucleotide/duplication Pyrococcus abyssi Orsay diphosphate + DNAn+1
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
 half-life: 5 h Pyrococcus abyssi