Crystallization (Comment) | Organism |
---|---|
crystal structure of Dpo4 in complex with North-methanocarba-dATP opposite dT | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
North-methanocarba-dATP | pH 7.5, 37°C | Saccharolobus solfataricus | |
0.0013 | - |
South-methanocarba-dATP | pH 7.5, 37°C | Saccharolobus solfataricus | |
0.0014 | - |
dATP | pH 7.5, 37°C | Saccharolobus solfataricus | |
0.0087 | - |
North-methanocarba-dATP | pH 7.5, 37°C | Saccharolobus solfataricus | |
0.01 | - |
dATP | pH 7.5, 37°C | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Saccharolobus solfataricus | Q97W02 | - |
- |
Saccharolobus solfataricus P2 | - |
- |
- |
Saccharolobus solfataricus P2 | Q97W02 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dATP + DNAn | - |
Saccharolobus solfataricus | ? | - |
? | |
dATP + DNAn | - |
Saccharolobus solfataricus P2 | ? | - |
? | |
North-methanocarba-dATP + DNAn | the role of sugar geometry during nucleotide selection is probed by the enzyme from Sulfolobus solfataricus using fixed conformation nucleotide analogues. The enzyme preferentially inserts North-methanocarba-dATP that locks the central ring into a RNA-type (C2'-exo, North) conformation near a C3'-endo pucker compared to a South-methanocarba-dATP that locks the central ring system into a (C3'-exo, South) conformation near a C2'-endo pucker | Saccharolobus solfataricus | ? | - |
? | |
North-methanocarba-dATP + DNAn | the role of sugar geometry during nucleotide selection is probed by the enzyme from Sulfolobus solfataricus using fixed conformation nucleotide analogues. The enzyme relatively tolerant to the substrate conformation: North-methanocarba-dATP that locks the central ring into a RNA-type (C2'-exo, North) conformation near a C3'-endo pucker or South-methanocarba-dATP that locks the central ring system into a (C3'-exo, South) conformation near a C2'-endo pucker | Saccharolobus solfataricus | ? | - |
? | |
North-methanocarba-dATP + DNAn | the role of sugar geometry during nucleotide selection is probed by the enzyme from Sulfolobus solfataricus using fixed conformation nucleotide analogues. The enzyme relatively tolerant to the substrate conformation: North-methanocarba-dATP that locks the central ring into a RNA-type (C2'-exo, North) conformation near a C3'-endo pucker or South-methanocarba-dATP that locks the central ring system into a (C3'-exo, South) conformation near a C2'-endo pucker | Saccharolobus solfataricus P2 | ? | - |
? | |
North-methanocarba-dATP + DNAn | the role of sugar geometry during nucleotide selection is probed by the enzyme from Sulfolobus solfataricus using fixed conformation nucleotide analogues. The enzyme preferentially inserts North-methanocarba-dATP that locks the central ring into a RNA-type (C2'-exo, North) conformation near a C3'-endo pucker compared to a South-methanocarba-dATP that locks the central ring system into a (C3'-exo, South) conformation near a C2'-endo pucker | Saccharolobus solfataricus P2 | ? | - |
? | |
South-methanocarba-dATP + DNAn | the role of sugar geometry during nucleotide selection is probed by the enzyme from Sulfolobus solfataricus using fixed conformation nucleotide analogues. The enzyme relatively tolerant to the substrate conformation: North-methanocarba-dATP that locks the central ring into a RNA-type (C2'-exo, North) conformation near a C3'-endo pucker or South-methanocarba-dATP that locks the central ring system into a (C3'-exo, South) conformation near a C2'-endo pucker | Saccharolobus solfataricus | ? | - |
? | |
South-methanocarba-dATP + DNAn | the role of sugar geometry during nucleotide selection is probed by the enzyme from Sulfolobus solfataricus using fixed conformation nucleotide analogues. The enzyme relatively tolerant to the substrate conformation: North-methanocarba-dATP that locks the central ring into a RNA-type (C2'-exo, North) conformation near a C3'-endo pucker or South-methanocarba-dATP that locks the central ring system into a (C3'-exo, South) conformation near a C2'-endo pucker | Saccharolobus solfataricus P2 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Dpo1 | - |
Saccharolobus solfataricus |
Dpo4 | - |
Saccharolobus solfataricus |
SSO2448 | locus name | Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0088 | - |
South-methanocarba-dATP | pH 7.5, 37°C | Saccharolobus solfataricus | |
0.014 | - |
North-methanocarba-dATP | pH 7.5, 37°C | Saccharolobus solfataricus | |
0.023 | - |
dATP | pH 7.5, 37°C | Saccharolobus solfataricus | |
0.08 | - |
North-methanocarba-dATP | pH 7.5, 37°C | Saccharolobus solfataricus | |
0.3 | - |
dATP | pH 7.5, 37°C | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Saccharolobus solfataricus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.8 | - |
South-methanocarba-dATP | pH 7.5, 37°C | Saccharolobus solfataricus | |
9.2 | - |
North-methanocarba-dATP | pH 7.5, 37°C | Saccharolobus solfataricus | |
11.7 | - |
North-methanocarba-dATP | pH 7.5, 37°C | Saccharolobus solfataricus | |
16.43 | - |
dATP | pH 7.5, 37°C | Saccharolobus solfataricus | |
30 | - |
dATP | pH 7.5, 37°C | Saccharolobus solfataricus |