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Literature summary for 2.7.7.7 extracted from

  • Walsh, J.M.; Ippoliti, P.J.; Ronayne, E.A.; Rozners, E.; Beuning, P.J.
    Discrimination against major groove adducts by Y-family polymerases of the DinB subfamily (2013), DNA Repair, 12, 713-722.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information pH 7.5, 50°C, Km is 0.71 mM for incorporation of dTTP in a 61mer template containing N6-furfuryl-deoxyadenosine Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxynucleoside triphosphate + DNAn Saccharolobus solfataricus the enzyme bypasses DNA adducts pyrrolo-deoxycytosine, dP, N6-furfuryl-deoxyadenosine, and 1,N6-ethenodeoxyadenosine in a process known as translesion synthesis diphosphate + DNAn+1
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?

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
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-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn the enzyme bypasses DNA adducts pyrrolo-deoxycytosine, dP, N6-furfuryl-deoxyadenosine, and 1,N6-ethenodeoxyadenosine in a process known as translesion synthesis Saccharolobus solfataricus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn the enzyme incorporates dTTP in a 61 mer template containing pyrrolo-deoxycytosine, dP, N6-furfuryl-deoxyadenosine, and 1,N6-ethenodeoxyadenosine (translesion synthesis) Saccharolobus solfataricus diphosphate + DNAn+1
-
?

Synonyms

Synonyms Comment Organism
Dpo4
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Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information pH 7.5, 50°C, kcat is 0.16/s for incorporation of dTTP in a 61mer template containing N6-furfuryl-deoxyadenosine Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
additional information
-
additional information pH 7.5, 50°C, kcat/Km is 0.0225/s * mM for incorporation of dTTP in a 61mer template containing N6-furfuryl-deoxyadenosine Saccharolobus solfataricus