Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.7 extracted from

  • Fiala, K.A.; Suo, Z.
    Sloppy bypass of an abasic lesion catalyzed by a Y-family DNA polymerase (2007), J. Biol. Chem., 282, 8199-8206.
    View publication on PubMed

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn an abasic lesion causes Dpo4 to switch from a normal to a very mutagenic mode of replication. Incorporation upstream of the abasic lesion is replicated error-free. Once Dpo4 encounters the lesion, synthesis became sloppy, with bypass products containing a myriad of mutagenic events. Incorporation of dAMP (29%) and dCMP (53%) opposite the abasic lesion at 37°C correlates exceptionally well with our kinetic results and demonstrates two dominant bypass pathways via the A-rule and the lesion loop-out mechanism. The percentage of overall frameshift mutations increases from 71% (37°C) to 87% (75°C) Saccharolobus solfataricus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn an abasic lesion causes the enzyme to switch from a normal to a very mutagenic mode of replication Saccharolobus solfataricus diphosphate + DNAn+1
-
?

Synonyms

Synonyms Comment Organism
Dpo4
-
Saccharolobus solfataricus

General Information

General Information Comment Organism
physiological function DNA damage that eludes cellular repair pathways can arrest the replication machinery and stall the cell cycle. This damage can be bypassed by the Y-family DNA polymerases Saccharolobus solfataricus