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Literature summary for 2.7.7.8 extracted from
- Preparation and properties of highly-purified Vibrio costicola polynucleotide phosphorylase (1985), Biochim. Biophys. Acta, 828, 29-38.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| KCl | salt is absolutely required for activity, maximal activity between 250 mM, inhibition above 1M | Salinivibrio costicola |  |
| NaCl | salt is absolutely required for activity, maximal activity at 250 mM, inhibition above 1 M | Salinivibrio costicola | |
| poly-L-lysine | 0.25 mg/ml, 78fold activation in the presence of 10 mM NaCl, 5fold activation in the presence of 250 mM NaCl | Salinivibrio costicola | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Acridine orange | inhibition of polymerization | Salinivibrio costicola | |
| NaCl | stimulates polymerization maximally at 250 mM, inhibition above | Salinivibrio costicola | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | divalent cation required, maximal activation at approx. 2 mM, Mg2+ is more effective than Mn2+ for polymerization, Mn2+ better activator in phosphorolytic reaction | Salinivibrio costicola | |
| Mn2+ | divalent cation required, Mg2+ more effective than Mn2+ for polymerization, Mn2+ better activator in phosphorolytic reaction | Salinivibrio costicola | |
| Na+ | NaCl stimulates polymerization maximally at250 mM, inhibition above | Salinivibrio costicola | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 72000 | - |
3 * 72000, SDS-PAGE in presence of 2-mercaptoethanol | Salinivibrio costicola |
| 220000 | - |
native PAGE | Salinivibrio costicola |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salinivibrio costicola | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| phosphocellulose, DEAE-Sephadex, Blue-Sepharose, Sephacryl s-300 | Salinivibrio costicola |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 99.01 | - |
- |
Salinivibrio costicola |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 100 mM NaCl, 0.1 mM EDTA, 0.1 mM dithiothreitol, 0.01 mM PMSF, 30% glycerol, 50 mM Tris-HCl, pH 8, at least 8 months, no loss of activity | Salinivibrio costicola |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| poly(C) + CDP | - |
Salinivibrio costicola | poly(C)+1 + phosphate | low poly(C) phosphorolysis activity | r | |
| poly(U)+ UDP | - |
Salinivibrio costicola | poly(U)+1 + phosphate | lower activity than with poly(A) | r | |
| RNAn + a nucleoside diphosphate | - |
Salinivibrio costicola | RNAn+1 + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 72000, SDS-PAGE in presence of 2-mercaptoethanol | Salinivibrio costicola |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Salinivibrio costicola |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9 | phosphorolysis | Salinivibrio costicola |
| 9.5 | - |
or above, polymerization | Salinivibrio costicola |
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