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Literature summary for 2.7.7.8 extracted from
- Purification and characterization of polynucleotide phosphorylase from cucumber (1985), Proc. Natl. Acad. Sci. USA, 82, 1311-1315.
General Stability
| General Stability | Organism |
|---|---|
| stable against several freezing and thawing cycles | Cucumis sativus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| phosphate | 0.5 mM, complete inhibition | Cucumis sativus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
UDP | pH 8.0, 30°C | Cucumis sativus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
3 * 50000, denaturing PAGE | Cucumis sativus |
| 150000 | - |
glycerol density gradient centrifugation | Cucumis sativus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cucumis sativus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, polyethlene glycol 6000, DEAE-Sepharose, QAE-Sephadex, blue-Sepharose, glycerol gradient centrifugation | Cucumis sativus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cotyledon | - |
Cucumis sativus | - |
| leaf | - |
Cucumis sativus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 34.08 | - |
incorporation of GDP | Cucumis sativus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -60°C, several months, no loss of activity | Cucumis sativus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| RNAn + a nucleoside diphosphate | activity in the absence of a primer, polymerization is stimulated by various polyribonucleotides or RNAs | Cucumis sativus | RNAn+1 + phosphate | - |
? | |
| RNAn + UDP | - |
Cucumis sativus | RNAn+1 + phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 50000, denaturing PAGE | Cucumis sativus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Cucumis sativus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.6 | - |
- |
Cucumis sativus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9.3 | approx. 60% of maximal activity at pH 7.5, approx. 70% of maximal activity at pH 9.3 | Cucumis sativus |
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Release 2023.1 (January 2023)
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