Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCl | almost complete inhibition of phosphorolysis and NDP-phosphate exchange reaction at approx. 150 mM | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
inner membrane | - |
Rattus norvegicus | - |
- |
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Rattus norvegicus | |
Mg2+ | maximal activation at 6 mM | Rattus norvegicus | |
Mn2+ | can partially replace Mg2+ in activation | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
manganese-RNA gel, DEAE-cellulose, ammonium sulfate, partially purified | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Storage Stability | Organism |
---|---|
-20°C, partially purified enzyme frozen and thawed after overnight storage, 30-40% loss of activity | Rattus norvegicus |
4°C, 1 week, 44% loss of phosphorolytic activity and 53% loss of NDP-phosphate exchange activity | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
poly(A)+1 + phosphate | - |
Rattus norvegicus | poly(A) + ADP | - |
ir | |
poly(C)+1 + phosphate | - |
Rattus norvegicus | poly(C) + ADP | - |
ir | |
poly(U)+1 + phosphate | - |
Rattus norvegicus | poly(U) + ADP | - |
ir | |
ribonucleoside 5'-diphosphate + phosphate | ADP preferred substrate for exchange, little or no reaction occurs with other nucleoside diphosphates | Rattus norvegicus | ribonucleoside 5'-diphosphate + phosphate | exchange reaction | ? | |
RNAn + a nucleoside diphosphate | polymerization of CDP | Rattus norvegicus | RNAn+1 + phosphate | - |
? | |
RNAn + a nucleoside diphosphate | ATP-phosphate exchange at one-third the rate observed with ADP | Rattus norvegicus | RNAn+1 + phosphate | - |
? | |
RNAn + a nucleoside diphosphate | polymerization of UDP | Rattus norvegicus | RNAn+1 + phosphate | - |
? | |
RNAn + a nucleoside diphosphate | polymerization of ADP | Rattus norvegicus | RNAn+1 + phosphate | - |
? | |
RNAn+1 + phosphate | phosphorolysis of poly(U) | Rattus norvegicus | RNAn + a nucleoside diphosphate | - |
ir | |
RNAn+1 + phosphate | phosphorolysis of poly(C) | Rattus norvegicus | RNAn + a nucleoside diphosphate | - |
ir | |
RNAn+1 + phosphate | phosphorolysis of RNA | Rattus norvegicus | RNAn + a nucleoside diphosphate | - |
ir | |
RNAn+1 + phosphate | enzyme has no nucleoside diphosphate-polymerization activity | Rattus norvegicus | RNAn + a nucleoside diphosphate | - |
ir |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
- |
Rattus norvegicus |