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Literature summary for 2.7.7.8 extracted from

  • See, Y.P.; Fitt, P.S.
    Partial purification and properties of rat liver mitochondrial polynucleotide phosphorylase (1972), Biochem. J., 130, 343-353.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
NaCl almost complete inhibition of phosphorolysis and NDP-phosphate exchange reaction at approx. 150 mM Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
inner membrane
-
Rattus norvegicus
-
-
mitochondrion
-
Rattus norvegicus 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Rattus norvegicus
Mg2+ maximal activation at 6 mM Rattus norvegicus
Mn2+ can partially replace Mg2+ in activation Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
manganese-RNA gel, DEAE-cellulose, ammonium sulfate, partially purified Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Storage Stability

Storage Stability Organism
-20°C, partially purified enzyme frozen and thawed after overnight storage, 30-40% loss of activity Rattus norvegicus
4°C, 1 week, 44% loss of phosphorolytic activity and 53% loss of NDP-phosphate exchange activity Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly(A)+1 + phosphate
-
Rattus norvegicus poly(A) + ADP
-
ir
poly(C)+1 + phosphate
-
Rattus norvegicus poly(C) + ADP
-
ir
poly(U)+1 + phosphate
-
Rattus norvegicus poly(U) + ADP
-
ir
ribonucleoside 5'-diphosphate + phosphate ADP preferred substrate for exchange, little or no reaction occurs with other nucleoside diphosphates Rattus norvegicus ribonucleoside 5'-diphosphate + phosphate exchange reaction ?
RNAn + a nucleoside diphosphate polymerization of CDP Rattus norvegicus RNAn+1 + phosphate
-
?
RNAn + a nucleoside diphosphate ATP-phosphate exchange at one-third the rate observed with ADP Rattus norvegicus RNAn+1 + phosphate
-
?
RNAn + a nucleoside diphosphate polymerization of UDP Rattus norvegicus RNAn+1 + phosphate
-
?
RNAn + a nucleoside diphosphate polymerization of ADP Rattus norvegicus RNAn+1 + phosphate
-
?
RNAn+1 + phosphate phosphorolysis of poly(U) Rattus norvegicus RNAn + a nucleoside diphosphate
-
ir
RNAn+1 + phosphate phosphorolysis of poly(C) Rattus norvegicus RNAn + a nucleoside diphosphate
-
ir
RNAn+1 + phosphate phosphorolysis of RNA Rattus norvegicus RNAn + a nucleoside diphosphate
-
ir
RNAn+1 + phosphate enzyme has no nucleoside diphosphate-polymerization activity Rattus norvegicus RNAn + a nucleoside diphosphate
-
ir

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
-
Rattus norvegicus