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Literature summary for 2.7.7.9 extracted from
- Oligomerization status, with the monomer as active species, defines catalytic efficiency of UDP-glucose pyrophosphorylase (2002), Biochem. J., 367, 295-300.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene Ugp, expression in Escherichia coli | Hordeum vulgare |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C99S | site-directed mutagenesis, half Vmax of wild-type, 12fold higher Km for diphosphate, altered diphosphate binding | Hordeum vulgare |
| additional information | amino acid exchanges in hydrophobic domain | Hordeum vulgare |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.034 | - |
diphosphate | recombinant wild-type, pH 7.6, 25°C | Hordeum vulgare |  |
| 0.065 | - |
UDP-glucose | recombinant wild-type, pH 7.6, 25°C | Hordeum vulgare | |
| 0.101 | - |
UDP-glucose | recombinant mutant C99S, pH 7.6, 25°C | Hordeum vulgare | |
| 0.407 | - |
diphosphate | recombinant mutant C99S, pH 7.6, 25°C | Hordeum vulgare | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
mixture of monomers, dimers and higher-order polymers, native PAGE and gel filtration, the monomer is the active form | Hordeum vulgare |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UTP + alpha-D-glucose 1-phosphate | Hordeum vulgare | key enzyme in biosynthesis of sucrose, cellulose, and other saccharides | diphosphate + UDP-glucose | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hordeum vulgare | Q43772 | gene Ugp | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native and recombinant wild-type, the latter from Escherichia coli to homogeneity | Hordeum vulgare |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Hordeum vulgare | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UTP + alpha-D-glucose 1-phosphate | - |
Hordeum vulgare | diphosphate + UDP-glucose | - |
r | |
| UTP + alpha-D-glucose 1-phosphate | key enzyme in biosynthesis of sucrose, cellulose, and other saccharides | Hordeum vulgare | diphosphate + UDP-glucose | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| UGPase | - |
Hordeum vulgare |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Hordeum vulgare |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
assay at | Hordeum vulgare |
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Release 2023.1 (January 2023)
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