Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | increases wild-type activity by about 25% | Hordeum vulgare |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Hordeum vulgare |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibitory: Tween-20 | Hordeum vulgare |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | Q43772 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Hordeum vulgare |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
similar activities of the wild-type UGPase in Tris, Hepes or Mops buffers. Half of the activity in 100 mM sodium phosphate. Compared with the wild-type UGPase the mutants KK127-128LL, C99S and LIV135-137NIN display reduced activities in all buffers. | Hordeum vulgare |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UTP + D-glucose-1-phosphate | - |
Hordeum vulgare | diphosphate + UDP-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | SDS-PAGE. Incubation of wild-type UGPase with phosphate or Tris buffers promote oligomerization, whereas Mops and Hepes completely dissociate the oligomers to monomers. Similar buffer effects for mutants KK127-128LL and C99S, small buffer effects for mutant LIV135-137NIN | Hordeum vulgare |