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Literature summary for 2.7.7.9 extracted from
- Identification, subcellular localization, biochemical properties, and high-resolution crystal structure of Trypanosoma brucei UDP-glucose pyrophosphorylase (2010), Glycobiology, 20, 1619-1630.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His6-tagged enzyme in Escherichia coli strain BL21 (DE3) | Trypanosoma brucei |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| purified recombinant enzyme, 17 mg/mL protein is mixed 1:1 with a crystallization buffer of 22% PEG 3350, 0.1 M ammonium sulfate, and 0.1 M Bis-Tris, pH 5.5, X-ray diffraction structure determination and analysis at 1.92 A resolution | Trypanosoma brucei |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics, overview | Trypanosoma brucei | |
| 0.053 | - |
UTP | pH 7.2, temperature not specified in the publication, recombinant enzyme | Trypanosoma brucei |  |
| 0.122 | - |
alpha-D-glucose 1-phosphate | pH 7.2, temperature not specified in the publication, recombinant enzyme | Trypanosoma brucei | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| glycosome | - |
Trypanosoma brucei | 20015 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | activates | Trypanosoma brucei | |
| Mg2+ | required, best at 10 mM | Trypanosoma brucei | |
| Mn2+ | activates | Trypanosoma brucei | |
| additional information | the enzyme is divalent cation-dependent, no activation by Zn2+ and Cu2+ | Trypanosoma brucei |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54507 | - |
x * 54507, sequence calculation | Trypanosoma brucei |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UTP + alpha-D-glucose 1-phosphate | Trypanosoma brucei | - |
diphosphate + UDP-glucose | - |
r | |
| UTP + alpha-D-glucose 1-phosphate | Trypanosoma brucei 427 | - |
diphosphate + UDP-glucose | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trypanosoma brucei | D3HTY9 | - |
- |
| Trypanosoma brucei 427 | D3HTY9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged enzyme from Escherichia coli strain by nickel affinity chromatography and gel filtration | Trypanosoma brucei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme is specific for alpha-D-glucose 1-phosphate, no activity with N-acetylgalactosamine 1-phosphate, N-acetylglucosamine 1-phosphate, or galactosamine 1-phosphate | Trypanosoma brucei | ? | - |
? | |
| additional information | the enzyme is specific for alpha-D-glucose 1-phosphate, no activity with N-acetylgalactosamine 1-phosphate, N-acetylglucosamine 1-phosphate, or galactosamine 1-phosphate | Trypanosoma brucei 427 | ? | - |
? | |
| UTP + alpha-D-glucose 1-phosphate | - |
Trypanosoma brucei | diphosphate + UDP-glucose | - |
r | |
| UTP + alpha-D-glucose 1-phosphate | - |
Trypanosoma brucei 427 | diphosphate + UDP-glucose | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 54507, sequence calculation | Trypanosoma brucei |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| UDP-glucose pyrophosphorylase | - |
Trypanosoma brucei |
| UGP | - |
Trypanosoma brucei |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7.5 | forward reaction | Trypanosoma brucei |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme catalyzes the last and reversible step of the de novo UDP-Glc biosynthetic pathway | Trypanosoma brucei |
| additional information | UDP-Glc is bound in a deep cleft located at the center of the catalytic domain that consists of highly conserved residues | Trypanosoma brucei |
| physiological function | UDP-glucose is the onyl precuror for UDP-galactose synthesis, which is one of the sugar nucleotides essentially required for the parasite's survival and infectivity. UDP-glucose is also the glucosyl donor for the unfolded glycoprotein glucosyltransferase involved in glycoprotein quality control in the endoplasmic reticulum and is the presumed donor for the synthesis of base J, i.e. beta-D-glucosyl-hydroxymethyluracil, a rare deoxynucleotide found in telomereproximal DNA in the bloodstream form of the parasite | Trypanosoma brucei |
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