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Literature summary for 2.7.7.B16 extracted from

  • Chemnitz Galal, W.; Pan, M.; Giulian, G.; Yuan, W.; Li, S.; Edwards, J.L.; Marino, J.P.; Kelman, Z.; Hurwitz, J.
    Formation of dAMP-glycerol and dAMP-Tris derivatives by Thermococcus kodakaraensis DNA primase (2012), J. Biol. Chem., 287, 16220-16229.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.B16

General Stability

General Stability Organism
stable to repeated freezing and thawing Thermococcus kodakarensis

Organism

Organism UniProt Comment Textmining
Thermococcus kodakarensis Q5JJ72 and Q5JJ73 Q5JJ72: small subunit and Q5JJ73: large subunit
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dATP + glycerol the products are formed by the p41 catalytic subunit alone and the p41-p46 complex in the absence of a DNA template. They are not formed with preparations containing the catalytically inactive p41 subunit Thermococcus kodakarensis dAMP-glycerol + diphosphate
-
 ?
dATP + Tris the products are formed by the p41 catalytic subunit alone and the p41-p46 complex in the absence of a DNA template. They are not formed with preparations containing the catalytically inactive p41 subunit Thermococcus kodakarensis dAMP-Tris + diphosphate
-
 ?
dCTP + glycerol the products are formed by the p41 catalytic subunit alone and the p41-p46 complex in the absence of a DNA template. They are not formed with preparations containing the catalytically inactive p41 subunit Thermococcus kodakarensis dAMP-glycerol + diphosphate
-
 ?
dCTP + Tris the products are formed by the p41 catalytic subunit alone and the p41-p46 complex in the absence of a DNA template. They are not formed with preparations containing the catalytically inactive p41 subunit Thermococcus kodakarensis dAMP-Tris + diphosphate
-
 ?
dGTP + glycerol the products are formed by the p41 catalytic subunit alone and the p41-p46 complex in the absence of a DNA template. They are not formed with preparations containing the catalytically inactive p41 subunit Thermococcus kodakarensis dGMP-glycerol + diphosphate
-
 ?
dGTP + Tris the products are formed by the p41 catalytic subunit alone and the p41-p46 complex in the absence of a DNA template. They are not formed with preparations containing the catalytically inactive p41 subunit Thermococcus kodakarensis dGMP-Tris + diphosphate
-
 ?
dTTP + glycerol the products are formed by the p41 catalytic subunit alone and the p41-p46 complex in the absence of a DNA template. They are not formed with preparations containing the catalytically inactive p41 subunit Thermococcus kodakarensis dTMP-glycerol + diphosphate
-
 ?
dTTP + Tris the products are formed by the p41 catalytic subunit alone and the p41-p46 complex in the absence of a DNA template. They are not formed with preparations containing the catalytically inactive p41 subunit Thermococcus kodakarensis dTMP-Tris + diphosphate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
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 assay at Thermococcus kodakarensis