Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion at 18°C, the structure provides the first three-dimensional description of the large subunit and its interaction with the small subunit | Saccharolobus solfataricus |
Protein Variants | Comment | Organism |
---|---|---|
F164E(PriS) | mutantion in small subunitPriS shows considerably weakened subunit association | Saccharolobus solfataricus |
F164G/I199K(PriS)_F142E/L163E(PriL) | double mutations of F164G/I199K in small subunit PriS and F142E/L163E in large subunit PriL abolishes the PriS-PriL interaction | Saccharolobus solfataricus |
G165I(PriS) | mutantion in small subunitPriS shows partial destabilization of the complex | Saccharolobus solfataricus |
additional information | mutant primase lacking the zinc-binding motifs has higher activity compared with the wild-type enzyme, as well as a bias toward shorter products of 30100 nucleotides | Saccharolobus solfataricus |
R84A/R85A(PriL) | mutation in large subunit shows a marked reduction in the size and amount of RNA product synthesized | Saccharolobus solfataricus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | contain one zinc ion in the small subunits. Residues Cys116, Cys119, Cys128 and Asp131 coordinate the zinc atom | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q9UWW1 and Q97Z83 | Q9UWW1: large subunit, Q97Z83: small subunit | - |
Saccharolobus solfataricus P2 | Q9UWW1 and Q97Z83 | Q9UWW1: large subunit, Q97Z83: small subunit | - |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
PriSL | - |
Saccharolobus solfataricus |
Sso core primase | - |
Saccharolobus solfataricus |