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Literature summary for 2.7.7.B16 extracted from

  • De Falco, M.; Fusco, A.; De Felice, M.; Rossi, M.; Pisani, F.M.
    The DNA primase of Sulfolobus solfataricus is activated by substrates containing a thymine-rich bubble and has a 3'-terminal nucleotidyl-transferase activity (2004), Nucleic Acids Res., 32, 5223-5230.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.B16

Activating Compound

Activating Compound Comment Organism Structure
additional information synthetic function is specifically activated by thymine-containing synthetic bubble structures that mimic early replication intermediates Saccharolobus solfataricus

Cloned(Commentary)

Cloned (Comment) Organism
small and large subunit are co-expressed in Escherichia coli as hexa-Histidine tagged proteins Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 1 * 36000 + 1 * 38000, SDS-PAGE Saccharolobus solfataricus
38000
-
 1 * 36000 + 1 * 38000, SDS-PAGE Saccharolobus solfataricus
80000
-
 gel filtration Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dNTP + n dNTP Saccharolobus solfataricus
-
 dN(pdN)n + n diphosphate
-
 ?
dNTP + n dNTP Saccharolobus solfataricus P2
-
 dN(pdN)n + n diphosphate
-
 ?
NTP + n NTP Saccharolobus solfataricus
-
 N(pN)n + n diphosphate
-
 ?
NTP + n NTP Saccharolobus solfataricus P2
-
 N(pN)n + n diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q9UWW1 and Q97Z83 Q9UWW1: large subunit, Q97Z83: small subunit
-
Saccharolobus solfataricus P2 Q9UWW1 and Q97Z83 Q9UWW1: large subunit, Q97Z83: small subunit
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharolobus solfataricus

Reaction

Reaction Comment Organism Reaction ID
dNTP + n dNTP = dN(pdN)n + n diphosphate (2) Saccharolobus solfataricus
a
NTP + n NTP = N(pN)n + n diphosphate (1) Saccharolobus solfataricus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dNTP + n dNTP
-
 Saccharolobus solfataricus dN(pdN)n + n diphosphate
-
 ?
dNTP + n dNTP utilizes poly-pyrimidine single-stranded DNA templates with low efficiency for de novo synthesis of RNA primers. Its synthetic function is specifically activated by thymine-containing synthetic bubble structures that mimic early replication intermediates. The enzyme is able to incorporate dATP on oligo(A)15/poly(dT) but with lower efficiency with respect to ATP. In addition, the Sso DNA primase is not able to elongate oligo(rA)15 when it is not annealed to poly(dT) or to initiate de novo synthesis on poly(dT) with dATP Saccharolobus solfataricus dN(pdN)n + n diphosphate
-
 ?
dNTP + n dNTP
-
 Saccharolobus solfataricus P2 dN(pdN)n + n diphosphate
-
 ?
dNTP + n dNTP utilizes poly-pyrimidine single-stranded DNA templates with low efficiency for de novo synthesis of RNA primers. Its synthetic function is specifically activated by thymine-containing synthetic bubble structures that mimic early replication intermediates. The enzyme is able to incorporate dATP on oligo(A)15/poly(dT) but with lower efficiency with respect to ATP. In addition, the Sso DNA primase is not able to elongate oligo(rA)15 when it is not annealed to poly(dT) or to initiate de novo synthesis on poly(dT) with dATP Saccharolobus solfataricus P2 dN(pdN)n + n diphosphate
-
 ?
additional information the enzyme although shows 3'-terminal nucleotidyl-transferase activity Saccharolobus solfataricus ?
-
 ?
additional information the enzyme although shows 3'-terminal nucleotidyl-transferase activity Saccharolobus solfataricus P2 ?
-
 ?
NTP + n NTP
-
 Saccharolobus solfataricus N(pN)n + n diphosphate
-
 ?
NTP + n NTP utilizes poly-pyrimidine single-stranded DNA templates with low efficiency for de novo synthesis of RNA primers. Its synthetic function is specifically activated by thymine-containing synthetic bubble structures that mimic early replication intermediates. The enzyme is able to incorporate dATP on oligo(rA)15/poly(dT) but with lower efficiency with respect to rATP. In addition, the Sso DNA primase is not able to elongate oligo(rA)15 when it is not annealed to poly(dT) or to initiate de novo synthesis on poly(dT) with dATP Saccharolobus solfataricus N(pN)n + n diphosphate
-
 ?
NTP + n NTP
-
 Saccharolobus solfataricus P2 N(pN)n + n diphosphate
-
 ?
NTP + n NTP utilizes poly-pyrimidine single-stranded DNA templates with low efficiency for de novo synthesis of RNA primers. Its synthetic function is specifically activated by thymine-containing synthetic bubble structures that mimic early replication intermediates. The enzyme is able to incorporate dATP on oligo(rA)15/poly(dT) but with lower efficiency with respect to rATP. In addition, the Sso DNA primase is not able to elongate oligo(rA)15 when it is not annealed to poly(dT) or to initiate de novo synthesis on poly(dT) with dATP Saccharolobus solfataricus P2 N(pN)n + n diphosphate
-
 ?

Subunits

Subunits Comment Organism
heterodimer 1 * 36000 + 1 * 38000, SDS-PAGE Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 assay at Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 assay at Saccharolobus solfataricus