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Literature summary for 6.3.1.2 extracted from

  • Harth, G.; Clemens, D.L.; Horwitz, M.A.
    Glutamine synthetase of Mycobacterium tuberculosis: extracellular release and characterization of its enzymatic activity (1994), Proc. Natl. Acad. Sci. USA, 91, 9342-9346.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
L-methionine sulfoximine
-
Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7
-
Glu
-
Mycobacterium tuberculosis
2.9
-
ATP Gln Mycobacterium tuberculosis

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular 30% of the total measurable enzyme activity is released into the culture medium Mycobacterium tuberculosis
-
-
phagosome Mycobacterium tuberculosis releases the enzyme into its phagosome in infected human monocytes Mycobacterium tuberculosis
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
12 * 58000, arranged in two superimpose hexagons, SDS-PAGE Mycobacterium tuberculosis
680000
-
gel filtration Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-Glu + NH4+ Mycobacterium tuberculosis roles of the enzyme in pathogenesis of Mycobacterium tuberculosis infection: 1. synthesis of Glu, that is a major component of the cell wall of pathogenic mycobacteria, 2. modulation of the NH4+ level in the Mycobacterium tuberculosis phagosome ?
-
?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Glu + NH4+
-
Mycobacterium tuberculosis ADP + phosphate + L-Gln
-
?
ATP + L-Glu + NH4+ roles of the enzyme in pathogenesis of Mycobacterium tuberculosis infection: 1. synthesis of Glu, that is a major component of the cell wall of pathogenic mycobacteria, 2. modulation of the NH4+ level in the Mycobacterium tuberculosis phagosome Mycobacterium tuberculosis ?
-
?
GTP + L-Glu + NH4+ 45% of the activity relative to ATP Mycobacterium tuberculosis GDP + phosphate + L-Gln
-
?

Subunits

Subunits Comment Organism
dodecamer 12 * 58000, arranged in two superimpose hexagons, SDS-PAGE Mycobacterium tuberculosis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
pH 7.0, 10 mM EDTA, 10 mM imidazole, complete loss of activity after 20 min Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
in presence of Mn2+ Mycobacterium tuberculosis
7.5
-
in presence of Mg2+ or Co2+ Mycobacterium tuberculosis