General Stability | Organism |
---|---|
treatment with 50 mM DTT for more than 1 h at 4°C, leads to some loss of activity, which is partially reversible. Further treatment leads to irreversible loss of activity | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
beta-Methylglutamate | - |
Rattus norvegicus | |
buthionine sulfoximine | - |
Rattus norvegicus | |
cystamine | completely reversible by DTT | Rattus norvegicus | |
D-3-amino-1-chloro-2-pentanone | highly potent irreversible inactivator | Rattus norvegicus | |
D-Glu | - |
Rattus norvegicus | |
gamma-Methylglutamate | - |
Rattus norvegicus | |
glutathione | - |
Rattus norvegicus | |
L-2-Amino-4-oxo-5-chloropentanoate | inactivation requires very low concentration, 0.003-0.006 mM, of Mg2+ or certain other divalent cations, L-Glu, but not D-Glu protects competitively against inactivation, protection is increased in the presence of ATP or ADP | Rattus norvegicus | |
L-3-Amino-1-chloro-2-pentanone | highly potent irreversible inactivator | Rattus norvegicus | |
methionine sulfoximine | - |
Rattus norvegicus | |
Prothionine sulfoximine | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
L-Cys | ATP, holoenzyme and recombinant heavy subunit | Rattus norvegicus | |
0.3 | - |
L-Cys | - |
Rattus norvegicus | |
1 | - |
L-2-aminobutanoate | - |
Rattus norvegicus | |
1.6 | - |
L-Glu | - |
Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
104000 | - |
- |
Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Glu + L-Cys | Rattus norvegicus | glutathione biosynthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-methylglutamate + L-Cys | i.e. 2-amino-2-methylpentanedioate | Rattus norvegicus | ADP + phosphate + alpha-methylglutamyl-L-Cys | - |
? | |
ATP + beta-aminoglutarate + L-Cys | i.e. 3-aminopentanedioate | Rattus norvegicus | ADP + phosphate + beta-aminoglutaryl-L-Cys | - |
? | |
ATP + beta-methylglutamate + L-Cys | i.e. 2-amino-3-methylpentanedioate | Rattus norvegicus | ADP + phosphate + beta-methylglutamyl-L-Cys | - |
? | |
ATP + D-Glu + L-Cys | - |
Rattus norvegicus | ADP + phosphate + D-Glu-L-Cys | - |
? | |
ATP + L-Glu + beta-chloro-L-Ala | - |
Rattus norvegicus | ADP + phosphate + gamma-L-Glu-L-beta-chloro-L-Ala | - |
? | |
ATP + L-Glu + DL-allylglycine | - |
Rattus norvegicus | ADP + phosphate + gamma-L-Glu-DL-allylglycine | - |
? | |
ATP + L-Glu + hydroxylamine | slow reaction rate | Rattus norvegicus | ADP + phosphate + gamma-L-Glu-hydroxylamine | - |
? | |
ATP + L-Glu + L-2-aminobutanoate | - |
Rattus norvegicus | ADP + phosphate + gamma-L-Glu-2-aminobutanoate | - |
? | |
ATP + L-Glu + L-Ala | - |
Rattus norvegicus | ADP + phosphate + gamma-L-Glu-L-Ala | - |
? | |
ATP + L-Glu + L-Cys | - |
Rattus norvegicus | ADP + phosphate + gamma-L-Glu-L-Cys | - |
? | |
ATP + L-Glu + L-Cys | glutathione biosynthesis | Rattus norvegicus | ? | - |
? | |
ATP + L-Glu + L-homocysteine | - |
Rattus norvegicus | ADP + phosphate + gamma-L-Glu-L-homocysteine | - |
? | |
ATP + L-Glu + L-norvaline | - |
Rattus norvegicus | ADP + phosphate + gamma-L-Glu-L-norvaline | - |
? | |
ATP + L-Glu + L-Thr | - |
Rattus norvegicus | ADP + phosphate + gamma-L-Glu-L-Thr | - |
? | |
ATP + N-methyl-L-glutarate + L-Cys | - |
Rattus norvegicus | ADP + phosphate + N-methyl-L-glutaryl-L-Cys | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.4 | - |
Rattus norvegicus |