EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
2.7.1.74 | 0.0236 |
- |
deoxythymidine |
at pH 8.0, temperature not specified in the publication |
762058 |
2.7.1.74 | 0.0581 |
- |
deoxythymidine |
at pH 8.0, temperature not specified in the publication |
762058 |
2.7.1.74 | 0.084 |
- |
deoxyuridine |
at pH 8.0, temperature not specified in the publication |
762058 |
2.7.1.74 | 0.14 |
- |
deoxyuridine |
at pH 8.0, temperature not specified in the publication |
762058 |
2.7.1.74 | 0.9 |
- |
2'-deoxyguanosine |
mutant enzyme R104M/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37°C |
702271 |
2.7.1.74 | 1.6 |
- |
2'-deoxyguanosine |
mutant enzyme R104L/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37°C |
702271 |
2.7.1.74 | 1.8 |
- |
fludarabine |
pH 7.6, 37°C, enzyme mutant S74E, with co-substrate ATP |
721556 |
2.7.1.74 | 2.6 |
- |
fludarabine |
pH 7.6, 37°C, enzyme mutant S74E, with co-substrate UTP |
721556 |
2.7.1.74 | 3 |
- |
fludarabine |
pH 7.6, 37°C, wild-type enzyme, with co-substrate ATP |
721556 |
2.7.1.74 | 4.3 |
- |
2'-deoxyadenosine |
mutant enzyme R104M/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37°C |
702271 |