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EC Number	Renatured (Commentary)	Reference
1.10.3.1	method one is to resuspend the proteins twice in 50 mM Tris-HCl buffer (pH 7.5) with 5 mM dithiothreitol, 2% (v/v) Triton X-100 and 5 mM NaCl, followed by centrifugation at 14000 x g for 5 min. Method two is to resuspend the proteins in one-tenth volume of its original culture of inclusion body isolation buffer containing 20 mM Tris-HCl and 1% (v/v) Triton X-100, pH 7.5, and then in 50 mM Tris-HCl, pH 8.0, each followed by centrifugation at 14000 x g for 5 min	712530

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Release 2023.1 (January 2023)