EC Number   |
|---|
    1.8.4.12 | batch method, crystals of the oxidized form are obtained by 32% PEG 4000, 0.8 M LiCl, and 0.1 M Tris HCl (pH 8.5), no crystal is obtained for wild type Xanthomonas campestris MsrB in its reduced form |
| 1.8.4.12 | batch method, using 30% PEG 400, 0.1 M Tris-HCl (pH 8.5), and 0.2 M Na-citrate |
| 1.8.4.12 | crystallized by the hanging-drop vapor-diffusion method. The crystals belong to the trigonal space group P3, with unit-cell parameters a = b = 136.096, c = 61.918 , and diffracted to 2.5 A resolution |
| 1.8.4.12 | hanging drop vapor diffusion method, crystals diffracting to 1.87 A resolution, the MsrB3 structure is determined by using X-ray crystallography |
| 1.8.4.12 | purified recombinant detagged MsrB domain containing SeMet39, hanging drop vapour-diffusion method, 15 mg/ml protein in 20 mM Tris, pH 8.5, 10% v/v glycerol, against a well solution containing 0.1 M sodium cacodylate, pH 6.5, 30% w/v PEG 4000, room temperature, X-ray diffraction structure determination and analysis at 1.8 A resolution, modeling |
| 1.8.4.12 | purified recombinant PilB mutant L38M/L41M, vapour diffusion method, 30 mg/ml protein in 20 mM HEPES, pH 7.5, and 100 mM NaCl, is mixed with well solution containing 0.1 M MES, pH 6.5, 0.2 M ammonium sulfate, 26% PEG 2000 monomethylester, and 25% glycerol, X-ray diffraction structure determination and analysis at 1.6 A resolution, multiwavelength anomalous dispersion at -170°C |
| 1.8.4.12 | selenomethionine-substituted peptide methionine sulfoxide reductase B domain, hanging drop vapour diffusion method in multiwell tissue-culture plates, 0.004 ml protein solution containing 75 mg/ml protein in 50 mM Tris-HCl, pH 8.0, mixed with 0.004 ml precipitant solution at 20°C, 3 days, X-ray diffraction structure determination and analysis at 1.8 A resolution |
| 1.8.4.12 | sitting drop vapor diffusion method, crystal structure of HpMsrAB C44S/C318S at 2.2 A, which shows that a linker region (Hpiloop, 193-205) between two domains interacts with each HpMsrA or HpMsrB domain via three salt bridges (E193-K107, D197-R103, and K200-D339) |
| 1.8.4.12 | to 2.3 A resolution, in presence and absence of dithiothreitol |
