EC Number   |
|---|
    2.4.1.15 | - |
| 2.4.1.15 | apoenzyme, enzyme in complex with UDP-alpha-D-glucose, and enzyme in complex with UDP-alpha-D-glucose and D-glucose 6-phosphate or UDP and alpha,alpha-trehalose 6-phosphate, sitting drop vapor diffusion method, using 0.2 M ammonium sulfate, 0.1 M Tris-HCl, pH 8.9, and 20% (w/v) PEG 3350 |
| 2.4.1.15 | apoenzyme, sitting drop vapor diffusion method, using 0.1 M Tris, pH 8.5, and 25% (w/v) PEG3350 |
| 2.4.1.15 | enzyme bound to either UDPG or UDP-alpha-D-glucose and D-glucose 6-phosphate or inhibitor validoxylamine A and UDP, hanging drop vapor diffusion method, using 0.1 M sodium malonate, pH 8.0, 0.1 M Tris, pH 8.0, and 30% (w/v) polyethylene glycol 1000 |
| 2.4.1.15 | enzyme bound to either UDPG or UDP-alpha-D-glucose and D-glucose 6-phosphate or inhibitor validoxylamine A and UDP, hanging drop vapor diffusion method, using 0.2 M lithium sulfate, 0.1 M Tris, pH 8.5, and 40% (w/v) polyethylene glycol 400 |
| 2.4.1.15 | in complex with UDP and D-glucose 6-phosphate, using 14% (w/v) PEG 4000, 0.15 M NaOAc, 0.1 M imidazole buffer (pH 7.5), and 200 mM NaCl |
| 2.4.1.15 | purified recombinant enzyme complexed with either substrate UDP-glucose or the inactive substrate analogue UDP-2-deoxy-2-fluoroglucose, hanging drop vapour diffusion method, 100 nl droplets of 10 mg/ml protein in 20 mM Tris-HCl, pH 8.0, and 200 mM NaCl, with either 25 mM UDP-glucose and 50 mM 1-deoxy-glucose 6-phosphate or 25 mM UDP-2-deoxy-2-fluoroglucose and 20 mM glucose 6-phosphate, precipitant solution: 30% w/v PEG 4000, 200 mM ammonium acetate, 100 mM Tris-HCl, pH 8.0, 36 h, 25% ethylene glycol as cryoprotectant, X-ray diffraction structure determination and analysis at 2.0 A resolution |
