EC Number   |
Protein Variants |
Reference |
|---|
    2.4.1.15 | D156G |
site-directed mutagenesis of a catalytic site residue, catalytically inactive Tps1 mutant variant |
736474 |
| 2.4.1.15 | D379A |
inactive |
757554 |
| 2.4.1.15 | E387A |
inactive |
757554 |
| 2.4.1.15 | E396A |
the mutant shows decreased catalytic activity compared to the wild type enzyme |
756013 |
| 2.4.1.15 | H223Y |
catalytically more active than wild-type enzyme |
488610 |
| 2.4.1.15 | K285A |
inactive |
757554 |
| 2.4.1.15 | K294A |
the mutant shows decreased catalytic activity compared to the wild type enzyme |
756013 |
| 2.4.1.15 | more |
activity of T6P synthase and the accumulation of trehalose during ethanol fermentation are significantly improved by overexpression of TPS1, and especially at 38°C cmpared to 30°C, no obvious difference occurs in the activity of T6P synthase and the trehalose concentration for wild-type strain 10151, and transformed strains 10151-URA3 and 10151-pI-RED1, indicating that the expression of URA3 and YAP1 genes, markers of the expression plasmid, does not affect the synthesis of trehalose |
719153 |
| 2.4.1.15 | more |
construction of a tps1DELTA and a tps1-156 deletion mutant |
736474 |
| 2.4.1.15 | more |
construction of N-loop exchange mutations of the otsA gene, the mutants show altered catalytic efficiencies at temperatures from 16 to 37°C compared to the wild-type enzyme, overview |
-, 718707 |
