EC Number |
Protein Variants |
Reference |
---|
2.7.1.74 | A100V/R104M/D133A |
12fold increase in ratio kcat/Km for deoxycytidine |
690954 |
2.7.1.74 | A100V/R104M/D133A |
site-directed mutagenesis, mutations alter the residues to the multisubstrate nucleoside kinase, EC 2.7.1.145, mutant shows 30fold increased kcat for deoxycytidine compared to the wild-type enzyme |
661731 |
2.7.1.74 | A100V/R104M/D133S |
6fold increase in ratio kcat/Km for deoxycytidine |
690954 |
2.7.1.74 | A100V/R104M/D133S |
the mutant shows exquisitely high activity for D-deoxycytidine and D-thymidine |
737326 |
2.7.1.74 | A100V/R104M/D133S/E196A |
the mutant shows low activity for D-deoxycytidine and D-thymidine |
737326 |
2.7.1.74 | A100V/R104M/D133S/E196L |
inactive |
737326 |
2.7.1.74 | A100V/R104M/D133S/F96D |
inactive |
737326 |
2.7.1.74 | A100V/R104M/D133S/F96Y |
the mutant shows low activity for D-deoxycytidine and D-thymidine |
737326 |
2.7.1.74 | A100V/R104M/D133S/W58E |
inactive |
737326 |
2.7.1.74 | A100V/R104M/D133S/W58V |
the mutant shows low activity for D-deoxycytidine and D-thymidine |
737326 |