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0.36
(R)-(-)-1-amino-2-propanol
25°C, pH 10, mutant enzyme G114A
-
0.2225
ethanol
at pH 8.8 and 37°C
0.00057 - 246
L-threonine
0.00802
n-butanol
at pH 8.8 and 37°C
additional information
additional information
-
0.061
L-serine
25°C, pH 10, wild-type enzyme
0.18
L-serine
25°C, pH 10, mutant enzyme G114A
1.6
L-serine
25°C, pH 10, mutant enzyme T178A
1.8
L-serine
25°C, pH 10, mutant enzyme I113A
0.00057
L-threonine
25°C, pH 10, mutant enzyme G114A
0.0026
L-threonine
25°C, pH 10, wild-type enzyme
0.0088
L-threonine
25°C, pH 10, mutant enzyme I113A
0.011
L-threonine
25°C, pH 10, mutant enzyme T178A
0.01129
L-threonine
at pH 8.8 and 37°C
0.0118
L-threonine
pH 7.5, 65°C, recombinant wild-type enzyme
0.013
L-threonine
-
pH 7.5, 65°C
0.043
L-threonine
25°C, pH 10, mutant enzyme D180A
1.1
L-threonine
-
metal-ion free enzyme
1.1
L-threonine
-
demetallized enzyme
1.5
L-threonine
-
pH 7.0, 70°C, recombinant enzyme
1.51
L-threonine
pH 7.5, 65°C, recombinant mutant E199A
1.6
L-threonine
-
at 50°C
2.5
L-threonine
pH 7.5, 65°C, recombinant mutant R204A
3.56
L-threonine
pH 9.0, 20°C
3.81
L-threonine
pH 9.0, 10°C
5.96
L-threonine
pH 9.0, 30°C
8.2
L-threonine
-
enzyme activated with 0.07 mM Cd2+
10.5
L-threonine
-
at pH 7.5
11.2
L-threonine
pH 9.0, 40°C
11.6
L-threonine
pH 10.0, 30°C, native enzyme
15.2
L-threonine
-
wild type enzyme, at pH 10.0 and 30°C
17.5
L-threonine
-
pH 10.0, 70°C
18
L-threonine
pH 9.0, 37°C
19.5
L-threonine
pH 9.0, 50°C
23.7
L-threonine
30°C, pH not specified in the publication, mutant enzyme T177A
25.3
L-threonine
30°C, pH not specified in the publication, wild-type enzyme
40.6
L-threonine
30°C, pH not specified in the publication, mutant enzyme Y136F
72.4
L-threonine
wild type enzyme, at pH 7.5 and 37°C
151.9
L-threonine
mutant enzyme M333E, at pH 7.5 and 37°C
211
L-threonine
-
enzyme activated with 0.25 mM Mn2+
221
L-threonine
-
enzyme saturated with 0.25 mM Mn2+
246
L-threonine
30°C, pH not specified in the publication, mutant enzyme S111A
0.0099
NAD+
pH 7.5, 65°C, recombinant wild-type enzyme
0.0099
NAD+
-
pH 7.5, 65°C, recombinant wild-type enzyme
0.014
NAD+
30°C, pH not specified in the publication, mutant enzyme S74A
0.0155
NAD+
30°C, pH not specified in the publication, mutant enzyme D179N
0.0196
NAD+
30°C, pH not specified in the publication, mutant enzyme S111A
0.0243
NAD+
30°C, pH not specified in the publication, mutant enzyme D179A
0.0257
NAD+
30°C, pH not specified in the publication, mutant enzyme T177A
0.0507
NAD+
30°C, pH not specified in the publication, wild-type enzyme
0.055
NAD+
-
pH 7.0, 70°C, recombinant enzyme
0.0615
NAD+
pH 7.5, 65°C, recombinant mutant E152A
0.097
NAD+
-
mutant enzyme T186N, at pH 10.0 and 30°C
0.1
NAD+
pH 10.0, 30°C, native enzyme
0.11
NAD+
-
metal-ion free enzyme
0.11
NAD+
-
demetallized enzyme
0.115
NAD+
-
mutant enzyme L80G, at pH 10.0 and 30°C
0.12
NAD+
-
wild type enzyme, at pH 10.0 and 30°C
0.152
NAD+
pH 7.5, 65°C, recombinant mutant E152T
0.156
NAD+
-
mutant enzyme G184A, at pH 10.0 and 30°C
0.163
NAD+
pH 7.5, 65°C, recombinant mutant E152C
0.18
NAD+
-
enzyme activated with 0.07 mM Cd2+
0.185
NAD+
-
pH 10.0, 70°C
0.216
NAD+
pH 7.5, 65°C, recombinant mutant E152Q
0.291
NAD+
pH 7.5, 65°C, recombinant mutant R204A
0.304
NAD+
pH 7.5, 65°C, recombinant mutant E199A
0.399
NAD+
pH 7.5, 65°C, recombinant mutant E152D
0.428
NAD+
30°C, pH not specified in the publication, mutant enzyme Y136F
0.6
NAD+
-
enzyme activated with 0.25 mM Mn2+
0.6
NAD+
-
enzyme saturated with 0.25 mM Mn2+
0.607
NAD+
pH 7.5, 65°C, recombinant mutant E152S
1.67
NAD+
wild type enzyme, at pH 7.5 and 37°C
2.06
NAD+
mutant enzyme R180K, at pH 7.5 and 37°C
additional information
additional information
-
kinetics
-
additional information
additional information
kinetic analysis of Glu152 mutants, overview
-
additional information
additional information
-
kinetic analysis of Glu152 mutants, overview
-