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1.1.1.105: all-trans-retinol dehydrogenase (NAD+)

This is an abbreviated version!
For detailed information about all-trans-retinol dehydrogenase (NAD+), go to the full flat file.

Word Map on EC 1.1.1.105

Reaction

all-trans-retinol-[cellular-retinol-binding-protein]
+
NAD+
=
all-trans-retinal-[cellular-retinol-binding-protein]
 +
NADH
 +
H+

Synonyms

ADH, alcohol dehydrogenase, all-trans retinol dehydrogenase, all-trans-retinol dehydrogenase, dehydrogenase, retinol, DHRS9, epidermal retinol dehydrogenase 2, LOC100185899, MDR, microsomal retinol dehydrogenase, mRDH1, NAD+-dependent retinoid-active short-chain dehydrogenase/reductase, P32, PDHC, photoreceptor retinol dehydrogenase isoform C, RDH-E2, RDH-like SDR, Rdh1, RDH10, RDH10a, RDH10b, RDH10c, RDH12, RDH16, RDH2, RDH7, RDH8, RDHE2, RDHE2S, RDHL, retinal reductase, retinene reductase, retinoid dehydrogenase/reductase, retinol dehydrogenase (vitamin A1), retinol dehydrogenase 10, retinol dehydrogenase 12, retinol dehydrogenase 2, retinol dehydrogenase 4, retinol dehydrogenase epidermal 2, retinol dehydrogenase epidermal 2-similar, RL-HSD, RoDH-4, RoDH-like 3alpha-hydroxysteroid dehydrogenase, RoDH4, SDR16C5, SDR16C6, XP_2120364

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.105 all-trans-retinol dehydrogenase (NAD+)

Cloned

Cloned on EC 1.1.1.105 - all-trans-retinol dehydrogenase (NAD+)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
both isoforms 1 and 2, expression in COS-7 cell with N-terminal His-tag
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cloning of Rdh7
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expressed in COS-1 cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli ER2566 cells
expressed in HEK-293 cells and Sf9 insect cells
expressed in HEK-293T cells
expressed in Sf9 insect cells and in HEK-293 cells
expression in CHO-K1 cells
expression in Escherichia coli
expression in HEK293 cells as FLAG-tagged fusions
expression in Sf9 cells, HepG2 and HEK293 cells
expression of His6-tagged RDH10 in Spodoptera frugiperda Sf9 insect cell membranes
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human RDHE2 exhibits a relatively low but reproducible activity when expressed in either HepG2 or HEK293 cells
human RDHE2S is unstable when expressed in HEK293 cells. RDHE2S protein produced in Sf9 cells is stable but has no detectable catalytic activity towards retinol
RDH-E2 is expressed in Sf9 insect cells as a fusion to the C-terminal His6-tag
RDH8 gene is located on 19p13, DNA and amino acid sequence determination and analysis, RDH8 genotping and phenotyping in Han Chinese population
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recombinant expression of C-terminally His6-tagged enzyme in Spodoptera frugiperda Sf9 cell microsomes, mitochondria from RDHE2S-expressing Sf9 cells exhibit a much lower activity than RDHE2S microsomes under the same assay conditions. Transient recombinant expression of C-terminally FLAG-tagged enzyme in HEK-293 cells. Quantitative RT-PCR enzyme expression analysis
recombinant expression of C-terminally His6-tagged enzyme in Spodoptera frugiperda Sf9 cell microsomes, no activity toward all-trans-retinol is detected using the microsomal and mitochondrial preparations of recombinant murine RDHE2 protein expressed in Spodoptera frugiperda Sf9 cells. Funtional transient recombinant expression of C-terminally FLAG-tagged enzyme in HEK-293 cells, the activity of RDHE2 resulted in a 3fold increase in RA biosynthesis relative to mock-transfected cells. Quantitative RT-PCR enzyme expression analysis
recombinant RoDH-4, expressed in microsomes of Sf9 insect cells
retinol dehydrogenase 4
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