1.1.1.136: UDP-N-acetylglucosamine 6-dehydrogenase
This is an abbreviated version!
For detailed information about UDP-N-acetylglucosamine 6-dehydrogenase, go to the full flat file.
Reaction
Synonyms
dehydrogenase, uridine diphosphoacetylglucosamine, gnaA, O180 O antigen, TviB, UDP-2-acetamido-2-deoxy-D-glucose:NAD oxidoreductase, UDP-GlcNAc 6-dehydrogenase, UDP-GlcNAc dehydrogenase, UDP-N-acetylglucosamine C-6 dehydrogenase, UDPacetylglucosamine dehydrogenase, UGlcNAcDH, uridine 5'-diphosphate-N-acetyl-D-glucosamine oxidoreductase, uridine diphosphoacetylglucosamine dehydrogenase, WbpA
ECTree
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Purification
Purification on EC 1.1.1.136 - UDP-N-acetylglucosamine 6-dehydrogenase
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recombinant C-terminally His6-tagged TviB from Escherichia coli strain BL21(DE3) by two steps of anion exchange chromatography, affinity chromatography, and gel filtration, inactive oligomeric forms of the enzym eis removed in gel filtration, to near homogeneity
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