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0.24
(S)-1-indanol
-
pH 7.4, 25°C
0.093
(S)-1-tetralol
-
pH 7.4, 25°C
0.0084
(S)-phenyl-1-butanol
-
pH 7.4, 25°C
0.089
1,4-Naphthoquinone
-
pH 7.4, 25°C
0.0052
1-phenylisatin
-
pH 7.4, 25°C
0.0038
11beta,17alpha,21-trihydroxy-4-pregnene-3,20-dione
-
-
0.0827
11beta,17alpha,21-trihydroxy-5alpha-pregnane-3,20-dione
-
-
0.0461
11beta,17alpha,21-trihydroxy-5beta-pregnane-3,20-dione
-
-
0.0621
13,14-dihydro-15-keto-prostaglandin F2
-
-
0.13
13,14-dihydro-15-keto-prostaglandin F2alpha
-
-
0.004 - 0.0196
16alpha-hydroxy-dehydroepiandrosterone
0.0014 - 0.0184
16beta-hydroxy-dehydroepiandrosterone
0.206
17-hydroxyprogesterone
-
-
0.0035 - 0.018
17alpha-hydroxypregnenolone
0.684
17beta-hydroxy-5alpha-androstane-3-one
-
-
0.231
17beta-hydroxy-5beta-androstane-3-one
-
-
0.2
2,3-heptanedione
-
pH 7.4, 25°C
0.55
2,3-Hexanedione
-
pH 7.4, 25°C
10
2,3-Pentanedione
-
pH 7.4, 25°C
0.0053
3beta,17alpha-dihydroxy-5-pregen-20-one
-
-
0.0015
4,4'-dimethylbenzil
-
pH 7.4, 25°C
1
4-benzoylpyridine
-
pH 7.4, 25°C
2.097
4-benzylpyridine
-
-
0.319
4-carboxybenzaldehyde
-
-
0.16
4-hexanoylpyridine
-
pH 7.4, 25°C
0.137
4-Nitroacetophenone
-
-
1.966 - 13
4-nitrobenzaldehyde
0.642
5-androsten-3beta-ol-17-one
pH 7.8, 30°C
0.0144 - 0.0147
5-androstene-3,17-dione
0.0167 - 0.0179
5alpha-Androstan-3beta-ol-17-one
0.028 - 1.043
5alpha-androstane-3,17-dione
0.016
5alpha-dihydrotestosterone
-
pH 7.4, 25°C
0.196 - 0.217
5alpha-pregnane-3,20-dione
0.116 - 0.12
5alpha-pregnane-3beta-ol-20-one
0.0049
5beta-androstan-17beta-ol-3-one
-
pH 7.4, 25°C
0.0079
5beta-androstan-3beta-ol-17-one
-
pH 7.4, 25°C
0.01 - 0.123
5beta-androstane-3,17-dione
0.0041
5beta-androstane-3beta,17beta-diol
-
pH 7.4, 25°C
0.0097
5beta-pregnan-20alpha-ol-3-one
-
pH 7.4, 25°C
0.008
5beta-Pregnan-3beta-ol-20-one
-
pH 7.4, 25°C
0.0029 - 1.725
5beta-pregnane-3,20-dione
0.008
5beta-pregnane-3beta,20alpha-diol
-
pH 7.4, 25°C
0.11 - 0.314
5beta-pregnane-3beta-ol-20-one
0.12
6-tert-butyl-2,3-epoxy-5-cyclohexene-1,4-dione
-
pH 7.4, 25°C
0.003
7alpha,27-dihydroxycholesterol
-
-
0.0032
7alpha-hydroxycholesterol
-
-
0.0036 - 2.408
9,10-phenanthrenequinone
0.024
all-trans-retinal
-
pH 7.4, 25°C
0.001
allopregnanolone
-
-
0.13
androst-5-ene-3beta-ol-17-one
-
NAD+ as cofactor
0.00016 - 0.00046
androstanediol
0.0703
androstenedione
-
-
0.005
benzil
-
pH 7.4, 25°C
0.465
D-glucuronic acid
-
-
1.07
D-glyceraldehyde
-
-
0.00009 - 0.2419
dehydroepiandrosterone
0.035
Dehydrolithocholic acid
-
pH 7.4, 25°C
0.02402
dehydrotomatidine
-
pH 7.2, 30°C
-
54
diacetyl
-
pH 7.4, 25°C
1.4
dimethyl-2-oxo-glutarate
-
pH 7.4, 25°C
0.036
hexanophenone
-
pH 7.4, 25°C
0.32
isatin
-
pH 7.4, 25°C
0.048
n-butyrophenone
-
pH 7.4, 25°C
0.0003
NADPH
-
pH 7.4, 25°C
0.00004 - 0.2313
pregnenolone
0.373
prostaglandin E1
-
-
0.0152
prostaglandin E2
-
-
21
Pyridine-4-aldehyde
-
pH 7.4, 25°C
0.0055 - 0.0068
stanolone
0.054
valerophenone
-
pH 7.4, 25°C
additional information
additional information
-
0.004
16alpha-hydroxy-dehydroepiandrosterone
mutant R195R, pH 7.4, 27°C
0.0064
16alpha-hydroxy-dehydroepiandrosterone
wild-type 3beta-HSD1, pH 7.4, 27°C
0.0148
16alpha-hydroxy-dehydroepiandrosterone
wild-type 3beta-HSD2, pH 7.4, 27°C
0.0196
16alpha-hydroxy-dehydroepiandrosterone
mutant R195P, pH 7.4, 27°C
0.0014
16beta-hydroxy-dehydroepiandrosterone
wild-type 3beta-HSD1, pH 7.4, 27°C
0.0015
16beta-hydroxy-dehydroepiandrosterone
mutant R195R, pH 7.4, 27°C
0.015
16beta-hydroxy-dehydroepiandrosterone
mutant R195P, pH 7.4, 27°C
0.0184
16beta-hydroxy-dehydroepiandrosterone
wild-type 3beta-HSD2, pH 7.4, 27°C
0.0035
17alpha-hydroxypregnenolone
27°C, pH 7.4
0.018
17alpha-hydroxypregnenolone
27°C, pH 7.4
1.966
4-nitrobenzaldehyde
-
-
13
4-nitrobenzaldehyde
-
pH 7.4, 25°C
0.0144
5-androstene-3,17-dione
-
placental microsomes
0.0147
5-androstene-3,17-dione
-
Sf9 cell homogenate
0.0167
5alpha-Androstan-3beta-ol-17-one
-
placental microsomes
0.0179
5alpha-Androstan-3beta-ol-17-one
-
Sf9 cell homogenate
0.028
5alpha-androstane-3,17-dione
-
pH 7.4, 25°C
1.043
5alpha-androstane-3,17-dione
-
-
0.196
5alpha-pregnane-3,20-dione
pH 7.8, 30°C
0.217
5alpha-pregnane-3,20-dione
-
-
0.116
5alpha-pregnane-3beta-ol-20-one
pH 7.8, 30°C
0.12
5alpha-pregnane-3beta-ol-20-one
-
NAD+ as cofactor
0.01
5beta-androstane-3,17-dione
-
pH 7.4, 25°C
0.123
5beta-androstane-3,17-dione
-
-
0.0029
5beta-pregnane-3,20-dione
-
pH 7.4, 25°C
0.246
5beta-pregnane-3,20-dione
-
-
1.725
5beta-pregnane-3,20-dione
pH 7.8, 30°C
0.11
5beta-pregnane-3beta-ol-20-one
-
NAD+ as cofactor
0.314
5beta-pregnane-3beta-ol-20-one
pH 7.8, 30°C
0.0036
9,10-phenanthrenequinone
-
pH 7.4, 25°C
2.408
9,10-phenanthrenequinone
-
-
0.00016
androstanediol
-
3betaHSD I
0.00046
androstanediol
-
3betaHSD III
0.00009
dehydroepiandrosterone
-
-
0.00012
dehydroepiandrosterone
-
3betaHSD VI
0.00014
dehydroepiandrosterone
-
3betaHSD I
0.00017
dehydroepiandrosterone
-
-
0.00042
dehydroepiandrosterone
-
pH 7.5, 37°C
0.00049
dehydroepiandrosterone
-
3betaHSD III
0.00059
dehydroepiandrosterone
-
37°C, pH 8.4
0.0007
dehydroepiandrosterone
-
wild-type, presence of 2-mercatoethanol pH 7.4
0.001
dehydroepiandrosterone
-
-
0.0016
dehydroepiandrosterone
-
microsomes
0.00164
dehydroepiandrosterone
mutant Q105M, pH 7.4, 27°C
0.0017
dehydroepiandrosterone
-
-
0.0018
dehydroepiandrosterone
-
37°C, pH 7.4
0.0024
dehydroepiandrosterone
-
type I
0.0028
dehydroepiandrosterone
-
mutant C72S, pH 7.4
0.0029
dehydroepiandrosterone
-
type II
0.0037
dehydroepiandrosterone
-
pH 7.4
0.0037
dehydroepiandrosterone
-
wild type enzyme
0.0037
dehydroepiandrosterone
27°C, pH 7.4
0.0037
dehydroepiandrosterone
-
27°C, pH 7.4
0.0037
dehydroepiandrosterone
-
wild-type, pH 7.4
0.0037
dehydroepiandrosterone
pH 7.4, 27°C, recombinant wild-type isozyme 3beta-HSD1
0.0037
dehydroepiandrosterone
pH 7.4, 27°C, recombinant wild-type isozyme 3beta-HSD1
0.0037
dehydroepiandrosterone
-
in 0.02 M potassium phosphate buffer, pH 7.4
0.0037
dehydroepiandrosterone
wild-type 3beta-HSD1, pH 7.4, 27°C
0.0037
dehydroepiandrosterone
wild-type, pH 7.4, 27°C
0.0037
dehydroepiandrosterone
pH 7.4, 27°C, wild-type enzyme
0.0044
dehydroepiandrosterone
-
mutant C72S, presence of 2-mercatoethanol pH 7.4
0.0045
dehydroepiandrosterone
-
mitochondria
0.0045
dehydroepiandrosterone
-
modified enzyme
0.005
dehydroepiandrosterone
-
-
0.0061
dehydroepiandrosterone
pH 7.4, 27°C, recombinant isozyme 3beta-HSD1 mutant Q240R
0.0075
dehydroepiandrosterone
-
mutant C83A, presence of 2-mercatoethanol pH 7.4
0.0077
dehydroepiandrosterone
mutant P195R, pH 7.4, 27°C
0.0077
dehydroepiandrosterone
mutant R195R, pH 7.4, 27°C
0.0082
dehydroepiandrosterone
mutant E192A, pH 7.4, 27°C
0.01
dehydroepiandrosterone
-
-
0.0107
dehydroepiandrosterone
mutant R195P, pH 7.4, 27°C
0.0107
dehydroepiandrosterone
mutant R195P, pH 7.4, 27°C
0.0107
dehydroepiandrosterone
pH 7.4, 27°C, chimeric R195P-1 mutant of the enzyme (3beta-HSD1)
0.011
dehydroepiandrosterone
-
27°C, pH 7.4, S123A mutant 3betaHSD1
0.0115
dehydroepiandrosterone
-
mutant C72F, presence of 2-mercatoethanol pH 7.4
0.0124
dehydroepiandrosterone
pH 7.4, 27°C, chimeric S194G-1 mutant of the enzyme (3beta-HSD1)
0.0127
dehydroepiandrosterone
-
mutant C72F, pH 7.4
0.0156
dehydroepiandrosterone
wild-type 3beta-HSD2, pH 7.4, 27°C
0.0156
dehydroepiandrosterone
wild-type, pH 7.4, 27°C
0.0156
dehydroepiandrosterone
pH 7.4, 27°C, wild-type enzyme
0.0168
dehydroepiandrosterone
-
mutant C111S, presence of 2-mercatoethanol pH 7.4
0.0171
dehydroepiandrosterone
-
mutant C83A, pH 7.4
0.0181
dehydroepiandrosterone
-
mutant C111S, pH 7.4
0.0237
dehydroepiandrosterone
-
mutant C111A, presence of 2-mercatoethanol pH 7.4
0.025
dehydroepiandrosterone
pH 7.4, 27°C, recombinant isozyme 3beta-HSD2 mutant Q105M
0.0268
dehydroepiandrosterone
-
mutant C111A, pH 7.4
0.0327
dehydroepiandrosterone
pH 7.4, 27°C, recombinant isozyme 3beta-HSD2 mutant R240Q
0.0351
dehydroepiandrosterone
mutant E192A, pH 7.4, 27°C
0.04
dehydroepiandrosterone
mutant Q105M, pH 7.4, 27°C
0.0405
dehydroepiandrosterone
pH 7.4, 27°C, recombinant isozyme 3beta-HSD1 mutant Q105M
0.042
dehydroepiandrosterone
-
27°C, pH 7.4, H156Y mutant
0.042
dehydroepiandrosterone
27°C, pH 7.4, H156Y mutant 3beta-HSD1
0.042
dehydroepiandrosterone
-
pH 7.4, H156Y mutant
0.0424
dehydroepiandrosterone
pH 7.4, 27°C, recombinant mutant H156Y
0.0424
dehydroepiandrosterone
mutant H156Y, pH 7.4, 27°C
0.047
dehydroepiandrosterone
-
27°C, pH 7.4
0.047
dehydroepiandrosterone
27°C, pH 7.4
0.047
dehydroepiandrosterone
-
27°C, pH 7.4
0.047
dehydroepiandrosterone
pH and temperature not specified in the publication
0.0473
dehydroepiandrosterone
pH 7.4, 27°C, recombinant wild-type isozyme 3beta-HSD2
0.0473
dehydroepiandrosterone
pH 7.4, 27°C, recombinant wild-type isozyme 3beta-HSD2
0.0643
dehydroepiandrosterone
mutant D61N, pH 7.4, 27°C
0.114
dehydroepiandrosterone
-
-
0.221
dehydroepiandrosterone
-
pH 8.5, 37°C
0.2419
dehydroepiandrosterone
pH 7.8, 30°C
0.18
menadione
-
pH 7.4, 25°C
0.0000047
NAD+
-
37°C, pH 7.25
0.0034
NAD+
-
wild type enzyme
0.0035
NAD+
-
modified enzyme
0.012
NAD+
-
wild-type, presence of 2-mercatoethanol pH 7.4
0.0153
NAD+
-
mutant C72S, pH 7.4
0.0166
NAD+
-
mutant C111S, presence of 2-mercatoethanol pH 7.4
0.019
NAD+
-
mutant C111S, pH 7.4
0.0194
NAD+
-
mutant C72S, presence of 2-mercatoethanol pH 7.4
0.0205
NAD+
mutant R195P, pH 7.4, 27°C
0.0205
NAD+
pH 7.4, 27°C, wild-type enzyme
0.0227
NAD+
-
mutant C111A, presence of 2-mercatoethanol pH 7.4
0.0244
NAD+
pH 7.4, 27°C, chimeric R195P-1 mutant of the enzyme (3beta-HSD1)
0.025
NAD+
-
27°C, pH 7.4, H156Y mutant
0.025
NAD+
27°C, pH 7.4, H156Y mutant 3beta-HSD1
0.0255
NAD+
-
mutant C111A, pH 7.4
0.0256
NAD+
pH 7.4, 27°C, recombinant isozyme 3beta-HSD1 mutant Q240R
0.0299
NAD+
-
mutant C83A, presence of 2-mercatoethanol pH 7.4
0.0333
NAD+
mutant E192A, pH 7.4, 27°C
0.034
NAD+
-
27°C, pH 7.4
0.0341
NAD+
-
wild-type, pH 7.4
0.0341
NAD+
pH 7.4, 27°C, recombinant wild-type isozyme 3beta-HSD1
0.0341
NAD+
-
in 0.02 M potassium phosphate buffer, pH 7.4
0.0341
NAD+
wild-type 3beta-HSD1, pH 7.4, 27°C
0.0341
NAD+
pH 7.4, 27°C, chimeric S194G-1 mutant of the enzyme (3beta-HSD1)
0.0351
NAD+
mutant E192A, pH 7.4, 27°C
0.036
NAD+
-
27°C, pH 7.4, S123A mutant 3betaHSD1
0.0372
NAD+
pH 7.4, 27°C, recombinant isozyme 3beta-HSD2 mutant R240Q
0.0388
NAD+
pH 7.4, 27°C, recombinant isozyme 3beta-HSD1 mutant Q105M
0.0468
NAD+
-
mutant C72F, pH 7.4
0.0476
NAD+
-
mutant C72F, presence of 2-mercatoethanol pH 7.4
0.0564
NAD+
mutant R195R, pH 7.4, 27°C
0.0614
NAD+
pH 7.4, 27°C, recombinant isozyme 3beta-HSD2 mutant Q105M
0.0643
NAD+
mutant D61N, pH 7.4, 27°C
0.0732
NAD+
-
mutant C83A, pH 7.4
0.086
NAD+
-
27°C, pH 7.4
0.0863
NAD+
pH 7.4, 27°C, recombinant wild-type isozyme 3beta-HSD2
0.0863
NAD+
wild-type 3beta-HSD2, pH 7.4, 27°C
0.0863
NAD+
pH 7.4, 27°C, wild-type enzyme
0.172
NAD+
-
cosubstrate pregnenolone, pH 8.5, 37°C
0.27
NAD+
-
cosubstrate dehydroepiandrosterone, pH 8.5, 37°C
0.0025
NADH
mutant E192A, pH 7.4, 27°C
0.0036
NADH
mutant R195P, pH 7.4, 27°C
0.0046
NADH
wild-type 3beta-HSD1, pH 7.4, 27°C
0.0055
NADH
mutant R195R, pH 7.4, 27°C
0.0065
NADH
mutant E192A, pH 7.4, 27°C
0.0072
NADH
mutant D61N, pH 7.4, 27°C
0.0126
NADH
wild-type 3beta-HSD2, pH 7.4, 27°C
0.0003
NADP+
-
pH 7.4, 25°C
0.00004
pregnenolone
-
3betaHSD VI
0.00008
pregnenolone
-
3betaHSD I
0.00047
pregnenolone
-
37°C, pH 7.25
0.001
pregnenolone
-
37°C
0.00103
pregnenolone
-
3betaHSD III
0.0011
pregnenolone
-
37°C
0.002
pregnenolone
-
mitochondria
0.0028
pregnenolone
27°C, pH 7.4
0.0032
pregnenolone
-
microsomes
0.0055
pregnenolone
-
type I
0.0088
pregnenolone
-
type II
0.02
pregnenolone
-
NAD+ as cofactor
0.023
pregnenolone
-
pH 8.5, 37°C
0.04534
pregnenolone
-
pH 7.2, 30°C
0.05
pregnenolone
27°C, pH 7.4
0.09
pregnenolone
pH 7.8, 30°C
0.2313
pregnenolone
pH 7.8, 30°C
0.0055
stanolone
-
3betaHSD I
0.0068
stanolone
-
3betaHSD III
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
kinetic analysis
-
additional information
additional information
-
kinetics recombinant wild-type isozyme and mutant H165Y, kinetics for the DELTA-5-3-ketosteroid isomerase, EC 5.3.3.1, activity
-
additional information
additional information
kinetics recombinant wild-type isozyme and mutant H165Y, kinetics for the DELTA-5-3-ketosteroid isomerase, EC 5.3.3.1, activity
-
additional information
additional information
kinetics recombinant wild-type isozyme and mutant H165Y, kinetics for the DELTA-5-3-ketosteroid isomerase, EC 5.3.3.1, activity
-
additional information
additional information
-
kinetics recombinant wild-type isozymes and mutant H165Y, kinetics for the DELTA-5-3-ketosteroid isomerase, EC 5.3.3.1, activity
-
additional information
additional information
kinetics recombinant wild-type isozymes and mutant H165Y, kinetics for the DELTA-5-3-ketosteroid isomerase, EC 5.3.3.1, activity
-
additional information
additional information
kinetics recombinant wild-type isozymes and mutant H165Y, kinetics for the DELTA-5-3-ketosteroid isomerase, EC 5.3.3.1, activity
-
additional information
additional information
kinetics, recombinant wild-type and mutant isozymes, overview, kinetics for the DELTA-5-3-ketosteroid isomerase, EC 5.3.3.1, activity
-
additional information
additional information
kinetics, recombinant wild-type and mutant isozymes, overview, kinetics for the DELTA-5-3-ketosteroid isomerase, EC 5.3.3.1, activity
-
additional information
additional information
-
kinetics, recombinant wild-type and mutant isozymes, overview, kinetics for the DELTA-5-3-ketosteroid isomerase, EC 5.3.3.1, activity
-