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1.1.1.159: 7alpha-hydroxysteroid dehydrogenase

This is an abbreviated version!
For detailed information about 7alpha-hydroxysteroid dehydrogenase, go to the full flat file.

Word Map on EC 1.1.1.159

Reaction

cholate
+
NAD+
=
3alpha,12alpha-dihydroxy-7-oxo-5beta-cholan-24-oate
+
NADH
+
H+

Synonyms

7-alpha-HSDH, 7-HSDH, 7alpha-HSD, 7alpha-HSDH, 7alpha-hydroxy steroid dehydrogenase, 7alpha-hydroxysteroid dehydrogenase, Bile acid-inducible protein, CA 7alpha-HSDH, Ca7alpha-HSDH, dehydrogenase, 7alpha-hydroxy steroid, J-1-1, NADP(H)-dependent 7alpha-HSDH, NADP(H)-dependent 7alpha-hydroxysteroid dehydrogenase, NADP+-dependent 7alpha-HSDH, nicotinamide adenine dinucleotide phosphate-dependent 7alpha-hydroxysteroid dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.159 7alpha-hydroxysteroid dehydrogenase

Temperature Stability

Temperature Stability on EC 1.1.1.159 - 7alpha-hydroxysteroid dehydrogenase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
15 - 65
the relative activity of the free enzyme drastically decreases at temperatures over 35°C, with minimal activity observed at 45°C. In contrast, the immobilized preparations are relatively stable at temperatures ranging from 15 to 35°C, with the relative activities of enzyme immobilized on the amino-epoxy resin and epoxy resin at 56.4% and 61.0%, respectively, after 2 h at 65°C
25
completely stable for 108 h
30
-
stable below
4 - 37
purified recombinant enzyme, no change in activity after incubation at 4°C or 25°C for 108 h, but dramatic decrease in activity at 37°C
60
-
10 min stable
75
-
30 min, presence of substrates, NAD+-dependent form stable
80
-
10 min, complete inactivation
additional information
-
the enzyme is thermostable