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0.19 - 55
D-fructose 6-phosphate
188.8 - 1458
D-fructose-6-phosphate
0.038 - 2
D-mannitol 1-phosphate
48.9 - 138
D-mannitol-1-phosphate
0.94
ethanol
-
cosubstrate: NAD+
0.8
hexitol phosphate
-
cosubstrate: NAD+
additional information
additional information
-
0.19
D-fructose 6-phosphate
recombinant enzyme, pH 7.0, 30°C
0.2
D-fructose 6-phosphate
cofactor NADH, 41°C, pH 7.5
0.25
D-fructose 6-phosphate
-
cosubstrate: NADH
0.3
D-fructose 6-phosphate
-
cosubstrate: NADH
0.3
D-fructose 6-phosphate
cofactor NADPH, 41°C, pH 7.5
0.54
D-fructose 6-phosphate
-
cosubstrate: NADH
1.66
D-fructose 6-phosphate
-
cosubstrate: NADH
2.1
D-fructose 6-phosphate
-
cosubstrate: NADH
2.1
D-fructose 6-phosphate
pH 7.1, 25°C, recombinant enzyme
3.2
D-fructose 6-phosphate
-
pH 10.0, 25°C, recombinant enzyme
13.6
D-fructose 6-phosphate
-
pH 7.0, 30°C
55
D-fructose 6-phosphate
pH 7.0, 37°C
188.8
D-fructose-6-phosphate
-
pH 7.0, 25°C
1458
D-fructose-6-phosphate
-
pH 7.0, 25°C, presence of 200 mM NaCl
0.038
D-mannitol 1-phosphate
-
cosubstrate: NAD+
0.05
D-mannitol 1-phosphate
-
cosubstrate: NAD+
0.055
D-mannitol 1-phosphate
-
cosubstrate: NAD+
0.0886
D-mannitol 1-phosphate
pH 7.5, temperature not specified in the publication
0.13
D-mannitol 1-phosphate
-
pH 7.1, 25°C, recombinant enzyme
0.15
D-mannitol 1-phosphate
-
cosubstrate: NAD+
0.23
D-mannitol 1-phosphate
pH 7.1, 25°C, recombinant enzyme
0.38
D-mannitol 1-phosphate
recombinant enzyme, pH 9.0, 30°C
2
D-mannitol 1-phosphate
-
cosubstrate: NAD+
48.9
D-mannitol-1-phosphate
-
pH 7.0, 25°C
138
D-mannitol-1-phosphate
-
pH 7.0, 25°C, presence of 200 mM NaCl
0.02
NAD+
recombinant enzyme, pH 9.0, 30°C
0.06
NAD+
-
cosubstrate: D-mannitol 1-phosphate
0.083
NAD+
-
cosubstrate: D-mannnitol 1-phosphate
0.11
NAD+
-
cosubstrate: D-mannitol 1-phosphate
0.2
NAD+
-
cosubstrate: hexitol phosphate
0.38
NAD+
-
cosubstrate: D-mannitol 1-phosphate
0.75
NAD+
pH 7.1, 25°C, recombinant enzyme
0.8
NAD+
-
pH 7.1, 25°C, recombinant enzyme
0.005
NADH
-
cosubstrate: D-fructose 6-phosphate
0.014
NADH
-
pH 10.0, 25°C, recombinant enzyme
0.016
NADH
-
cosubstrate: D-fructose 6-phosphate
0.016
NADH
pH 7.1, 25°C, recombinant enzyme
0.03
NADH
-
higher affinity for NADH
0.099
NADH
-
cosubstrate: D-fructose 6-phosphate
0.14
NADH
-
cosubstrate: D-fructose 6-phosphate
5.4
NADH
-
pH 7.0, 25°C, presence of 200 mM NaCl
16
NADH
recombinant enzyme, pH 7.0, 30°C
56.9
NADH
-
pH 7.0, 25°C, presence of 200 mM NaCl
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
kinetic isotope effects, overview
-
additional information
additional information
-
kinetic isotope effects, overview
-
additional information
additional information
-
steady-state kinetics and kinetic mechanism, binding of D-mannitol 1-phosphate and NAD+ is random, whereas D-fructose 6-phosphate binds only after NADH has bound to the enzyme. Hydride transfer is rate-determining for D-mannitol 1-phosphate oxidation by AfM1PDH. AfM1PDH behaves kinetically as a fructose 6-phosphate reductase, overview
-