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1.1.1.209: 3(or 17)alpha-hydroxysteroid dehydrogenase

This is an abbreviated version!
For detailed information about 3(or 17)alpha-hydroxysteroid dehydrogenase, go to the full flat file.

Word Map on EC 1.1.1.209

Reaction

androsterone
+
NAD(P)+
=
5alpha-androstane-3,17-dione
 +
NAD(P)H
 +
H+

Synonyms

3(17)alpha-hydroxysteroid dehydrogenase, 3beta- and 17beta-hydroxysteroid dehydrogenase, AKR1C21, dehydrogenase, 3(17)alpha-hydroxy steroid, More

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.209 3(or 17)alpha-hydroxysteroid dehydrogenase

Engineering

Engineering on EC 1.1.1.209 - 3(or 17)alpha-hydroxysteroid dehydrogenase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
G225P/G226P
reduced affinity (Km) for both 3alpha- and 17alpha-hydroxysteroid substrates by up to 160fold, shows high selectivity for 5beta-pregnane-3alpha,20alpha-diol in the oxidation of the 3alpha-hydroxy group and, conversely, broad specificity for both 17alpha- and 17beta-hydroxysteroids
Q222N
mutation of residue involved in forming the safety belt for binding of the coenzyme. Kinetic constants for 3-hydroxy/3-ketosteroids and 17-hydroxy/ketosteroids similar to wild type
Y224D
mutation reduces the Km value for NADP(H) by up to 80fold and completely reverses the 17alpha stereospecificity of the enzyme
Y224F
mutation of residue involved in forming the safety belt for binding of the coenzyme. Kinetic constants for 3-hydroxy/3-ketosteroids and 17-hydroxy/ketosteroids similar to wild type