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0.05617
5-azido-UDP-glucose
-
22°C, pH 8.7
0.058
aldehyde intermediate
-
-
-
0.00726 - 2.21
UDP-alpha-D-glucose
additional information
additional information
-
0.006
NAD+
37°C, pH 10.5
0.042
NAD+
-
isoform Ugd3, pH 8.7, 22°C
0.043
NAD+
-
isoform Ugd2, pH 8.7, 22°C
0.044
NAD+
-
isoform Ugd4, pH 8.7, 22°C
0.048
NAD+
mutant A104L, K0.5 value, Hill coefficient 1, pH 7.5, 25°C
0.0722
NAD+
Saccharum spp.
-
pH 8.4, 25°C
0.09
NAD+
mutant A136M, Hill coefficient, pH 7.5, 25°C
0.1
NAD+
mutant E161Q, pH 8.7, 25°C
0.132
NAD+
-
pH 8.8, 25°C
0.133
NAD+
-
wild-type, pH 8.7, 22°C
0.135
NAD+
-
mutant E141Q
0.187
NAD+
-
mutant E145Q
0.31
NAD+
-
pH 9.5, 37°C, recombinant wild-type enzyme
0.333
NAD+
K0.5 value, Hill coefficient 0.8, pH 7.5, 25°C
0.35 - 0.62
NAD+
-
pH 9.5, 37°C, recombinant wild-type enzyme, from different strains
0.35 - 0.62
NAD+
-
pH 9.5, 37°C, recombinant wild-type enzyme, with 0.025-1.0 mM ATP
0.38
NAD+
pH 8.7, 37°C, recombinant enzyme
0.384
NAD+
wild-type, pH 8.7, 25°C
0.401
NAD+
-
mutant A222Q/S233G, pH 8.7, 22°C
0.42
NAD+
-
wild-type, pH 7.4, 22°C
0.43
NAD+
-
pH 9.5, 37°C, recombinant mutant K323A
0.5
NAD+
-
pH 9.5, 37°C, recombinant mutant Y71F
0.53
NAD+
-
mutant K339A, pH 7.4, 22°C
0.53
NAD+
wild-type, pH 8.7, 30°C, Hill-coefficient 1.5
0.646
NAD+
pH 7.4, 37°C, recombinant His-tagged wild-type enzyme
0.7
NAD+
wild-type, pH 8.7, 25°C
0.78
NAD+
wild-type, Hill coefficient 0.73, pH 7.5, 25°C
0.897
NAD+
pH 7.4, 37°C, recombinant His-tagged mutant T325A
0.942
NAD+
wild-type, pH 7.5, 25°C, Hill coefficient 0.74
1.084
NAD+
pH 7.4, 37°C, recombinant His-tagged mutant T325D
1.16
NAD+
wild-type, K0.5 value, Hill coefficient 0.69, pH 7.5, 25°C
2.1
NAD+
-
mutant K339A, pH 7.4, 22°C
2.92
NAD+
mutant K94E, pH 7.5, 25°C
6.3
NAD+
mutant K94E, pH 8.7, 25°C
0.017
UDP
-
wild-type, pH 8.7, 22°C
0.017
UDP
-
wild type enzyme, at pH 8.7 and 25°C
0.98
UDP
-
mutant A222Q/S233G, pH 8.7, 22°C
0.98
UDP
-
mutant enzyme A222Q/S233G, at pH 8.7 and 25°C
0.00726
UDP-alpha-D-glucose
mutant A136M, Hill coefficient, pH 7.5, 25°C
0.0076
UDP-alpha-D-glucose
wild-type, K0.5 value, Hill coefficient 1, pH 7.5, 25°C
0.0094
UDP-alpha-D-glucose
mutant A104L, K0.5 value, Hill coefficient 1, pH 7.5, 25°C
0.0097
UDP-alpha-D-glucose
wild-type, Hill coefficient 1, pH 7.5, 25°C
0.015
UDP-alpha-D-glucose
pH 7.4, 37°C, recombinant His-tagged mutant T325D
0.016
UDP-alpha-D-glucose
wild-type, pH 7.5, 25°C
0.021
UDP-alpha-D-glucose
-
pH 8.8, 25°C
0.025
UDP-alpha-D-glucose
wild-type, pH 8.7, 25°C
0.034
UDP-alpha-D-glucose
pH 7.4, 37°C, recombinant His-tagged wild-type enzyme
0.035
UDP-alpha-D-glucose
wild-type, pH 8.7, 25°C
0.048
UDP-alpha-D-glucose
pH 7.4, 37°C, recombinant His-tagged mutant T325A
0.055
UDP-alpha-D-glucose
mutant E161Q, pH 8.7, 25°C
0.055
UDP-alpha-D-glucose
Hill coefficient 1, pH 7.5, 25°C
0.0958
UDP-alpha-D-glucose
pH 8.4, 30°C
0.19
UDP-alpha-D-glucose
pH 8.7, 25°C
0.23
UDP-alpha-D-glucose
wild-type, pH 8.7, 30°C, Hill-coefficient 0.89
0.269
UDP-alpha-D-glucose
mutant K94E, pH 7.5, 25°C
0.32
UDP-alpha-D-glucose
37°C, pH 10.5
0.42
UDP-alpha-D-glucose
50°C, pH 10.5
0.47
UDP-alpha-D-glucose
pH 8.7, 37°C, recombinant enzyme
1.28
UDP-alpha-D-glucose
70°C, pH 10.5
2.21
UDP-alpha-D-glucose
mutant K94E, pH 8.7, 25°C
0.0092
UDP-glucose
-
wild-type, pH 7.4, 22°C
0.011
UDP-glucose
pH 7.4, 22°C
0.017
UDP-glucose
pH 8.7, 25°C
0.01703
UDP-glucose
-
22°C, pH 8.7
0.0187
UDP-glucose
Saccharum spp.
-
pH 8.4, 25°C
0.02
UDP-glucose
-
wild-type
0.022
UDP-glucose
-
pH 8.7, 25°C
0.022
UDP-glucose
-
mutant K339A, pH 7.4, 22°C
0.059
UDP-glucose
-
mutant T118A
0.06
UDP-glucose
-
mutant E141Q
0.12
UDP-glucose
pH 7.5, 22°C
0.123
UDP-glucose
-
isoform Ugd2, pH 8.7, 22°C
0.125
UDP-glucose
-
mutant E145Q
0.171
UDP-glucose
-
isoform Ugd4, pH 8.7, 22°C
0.2
UDP-glucose
pH 8.7, 22°C
0.335
UDP-glucose
-
isoform Ugd3, pH 8.7, 22°C
0.38
UDP-glucose
-
20°C, pH 8, UDPGDH-A
0.4
UDP-glucose
pH 7.5, 22°C
0.87
UDP-glucose
-
pH 8.7, 30°C
0.95
UDP-glucose
-
20°C, pH 8, UDPGDH-B
1.5
UDP-glucose
-
mutant K339A, pH 7.4, 22°C
0.015
UDPglucose
-
pH 9.4
0.035
UDPglucose
-
pH 8.6
0.0756
UDPglucose
-
native enzyme
1
UDPglucose
-
dissociated enzyme
8.4
UDPglucose
-
recombinant glutathione-S-transferase fusion protein
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
pH-dependence of Km
-
additional information
additional information
-
KM increase at pH higher than 9.0
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten kinetic, cooperative kinetic behavior occurs in the hexameric enzyme
-