1.1.1.237: hydroxyphenylpyruvate reductase

This is an abbreviated version!
For detailed information about hydroxyphenylpyruvate reductase, go to the full flat file.

Reaction

Synonyms

4-hydroxyphenylpyruvate reductase, At1g12940, At1g79870, H(P)PR, HPPR, HPPR2, HPPR3, HPR2, HPR3, HPRP, hydroxy(phenyl)pyruvate reductase, hydroxyphenylpyruvate reductase, hydroxyphenylpyruvic acid reductase, Ppr, pprA

ECTree

     1 Oxidoreductases

         1.1 Acting on the CH-OH group of donors

             1.1.1 With NAD⁺ or NADP⁺ as acceptor

                1.1.1.237 hydroxyphenylpyruvate reductase

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Results	in table
5	Activating Compound
25	AA Sequence
1	CAS Registry Number
10	Cloned(Commentary)
18	Cofactor
1	Crystallization (Commentary)
7	Expression
25	General Information
1	General Stability
14	Inhibitors
12	kcat/KM [mM/s]
22	KM Value [mM]
5	Localization
9	Molecular Weight [Da]
13	Natural Substrates/ Products (Substrates)
22	Organism
8	Pathway
2	PDB ID
4	pH Optimum
1	pI Value
5	Protein Variants
5	Purification (Commentary)
2	Reaction
3	Reaction Type
21	Reference
38	Source Tissue
2	Specific Activity [micromol/min/mg]
2	Storage Stability
53	Substrates and Products (Substrate)
8	Subunits
33	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
13	Turnover Number [1/s]

Crystallization

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Crystallization on EC 1.1.1.237 - hydroxyphenylpyruvate reductase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

apoenzyme and enzyme in complex with NADP+, sitting drop vapour diffusion method, mixing of 0.001 ml of 6 mg/ml protein or 15 mg/ml protein with 2 mM NADP+ in 50 mM Tris-HCl, pH 7.0, with 0.001 ml of well solution containing 30% 2-methyl-2,4-pentanediol, 0.2 M NaCl, 0.1 M Tris-HCl, pH 7.5 at 4°C, or 20% PEG 1000, 0.1 M imidazole, pH 7.5, 4 mM 4-hydroxyphenylpyruvate, 0.2 mM DTT at 26°C, leading to one-crystal clusters or tetragonal bipyramid-shaped crystals, X-ray diffraction structure determination and analysis at 1.47 A and 2.2 A, respectively, modeling

Coleus scutellarioides

Q65CJ7

723825