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1.1.1.267: 1-deoxy-D-xylulose-5-phosphate reductoisomerase

This is an abbreviated version!
For detailed information about 1-deoxy-D-xylulose-5-phosphate reductoisomerase, go to the full flat file.

Word Map on EC 1.1.1.267

Reaction

2-C-methyl-D-erythritol 4-phosphate
+
NADP+
=
1-deoxy-D-xylulose 5-phosphate
+
NADPH
+
H+

Synonyms

1-deoxy-D-xylulose 5-phosphate isomeroreductase, 1-deoxy-D-xylulose 5-phosphate reductoisomerase, 1-deoxy-D-xylulose-5-phosphate, 1-deoxy-D-xylulose-5-phosphate reductoisomerase, 1-deoxy-D-xylulose-5-phosphate synthase, 1-deoxyxylulose 5-phosphate reductoisomerase, 1-deoxyxylulose-5-phosphate reductoisomerase, 2-C-methyl-D-erythritol 4-phosphate synthase, 2C-methyl-D-erythritol 4-phosphate synthase, 2C-methyl-D-erythritol-4-phosphate synthase, AaDXR, CaDXR, deoxyxylulose 5-phosphate reductoisomerase, deoxyxylulosephosphate reductoisomerase, DOXP reductoisomerase, DOXP-reductoisomerase, DXP reductoisomerase, DXP-reductoisomerase, DXR, DXR1, DzDXR, EcDXR, FtIspC, IspC, MEP synthase, meps, methylerythritol phosphate synthase, MtbIspC, MtDXR, Os01g01710, OsDXR, PdDXR, PfDXR, PtDXR, Rv2870c, SaDXR, VvDxr, YpIspC

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.267 1-deoxy-D-xylulose-5-phosphate reductoisomerase

Purification

Purification on EC 1.1.1.267 - 1-deoxy-D-xylulose-5-phosphate reductoisomerase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by immobilized metal affinity chromatography on a Co column, ca. 90% pure
-
by immobilized metal affinity chromatography on a Co column, followed by anion exchange chromatography, gel filtration and ultrafiltration
-
of recombinant protein
recombinant C-terminally His-tagged enzyme 8.7fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultracentrifugation and dialysis, followed by anion exchange and hydroxylapatite chromatography, affinity chromatography, and again dialysis
recombinant His-tagged enzyme by nickkel affinity chromatography and gel filtration
recombinant His-tagged enzyme from Escherichia coli strain BL21 by metal ion affinity chromatography, and gel filtration, to 98% purity
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and desalting gel filtration
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to over 90% purity
-
recombinant His-tagged enzyme from overexpressing strain BL21 by nickel affinity chromatography
-
recombinant His-tagged enzyme in the pseudomature form without plastid-targeting sequence 62fold from Escherichia coli
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by metal ion affinity chromatography
-
recombinant His6-tagged enzyme from Escherichia coli BL21-CodonPlus strain by nickel affinity chromatography
-
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli by metal ion affinity chromatography
-
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and gel filtration, followed by tag cleavage through TEV protease, and another step of nickel affinity chromatography, and terminated by gel filtration
recombinant protein
recombinant tagged enzyme from Escherichia coli strain BL21 Codon Plus (DE3)-RIL by metal affinity chromatography and ultrafiltration