1.1.1.282: quinate/shikimate dehydrogenase

This is an abbreviated version!
For detailed information about quinate/shikimate dehydrogenase, go to the full flat file.

Word Map on EC 1.1.1.282

Reaction

L-quinate
+
NAD(P)+
=
3-dehydroquinate
+
NAD(P)H
+
H+

Synonyms

cgl0424, cgR_0495, dehydroquinate dehydratase-shikimate dehydrogenase, More, Poptr2, Poptr3, Poptr4, QSDH, qsuD, quinate/shikimate 5-dehydrogenase, quinate/shikimate dehydrogenase, rifI, RifI2, SDH, SDH/QDH, sdhL, shikimate/quinate dehydrogenase, YdiB

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.282 quinate/shikimate dehydrogenase

Cloned

Cloned on EC 1.1.1.282 - quinate/shikimate dehydrogenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
five putative SDH-encoding genes amplified and cloned into either a modified pET28a vector or the p15TVLIC vector, a ligation-independent vector due to incompatible cloning sites between the insert and pET28.3. Expressed in either the Escherichia coli strain BL21 gold (p15TV-LIC constructs) or the Escherichia coli BL21CodonPlus (pET28.3constructs)
gene cgR_0495, recombinant expression
gene qsuD, recombinant expression
gene qsuD, recombinant expression in Escherichia coli
gene ydiB, recombinant overexpression in Escherichia coli strain PB12 causing a 500% increase in the molar yield of quinate and results in a 152% increase in quinate (mol/mol) relative to shikimate, with a sharp decrease in shikimate production. Transcriptomic analysis of PB12.SA22 and ydiB derivative strains
-
recombinant expression of His6-tagged isozymes in Escherichia coli strain M15
-
recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21 gold
ydiB gene, expression in Escherichia coli BL21(DE3)